Purification of Toad (Bufo japonicus) Gonadotropins and Development of Their Homologous Radioimmunoassays : Endocrinology

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概要

• 論文の詳細を見る

• We obtained three gonadotropin fractions with different electrophoretic mobilities named B1D, B3D and B5D from a glycoprotein fraction of toad (Bufo japonicus) pituitaries by cation exchange chromatography using the fast protein liquid chromatography (FPLC) system, chromatofocusing and gel filtration using the FPLC system. Gonadotropin activity was monitored by two radioreceptor assay (RRA) systems, one using bullfrog testis and bullfrog LH as the source of receptor and radioligand respectively, and the other using toad testis and bullfrog FSH respectively. Although, LH/FSH specificity was not complete in these RRAs, the fraction B1D showed a higher potency in LH-RRA than in FSH-RRA, while B3D and B5D showed lower potencies in LH-RRA activity than in FSH-RRA. Furthermore, B1D had an activity to release androgen from the toad testis, while B3D and B5D had slight activities. All these fractions stimulated accumulation of cAMP in testis slices of the toad in vitro. These results suggest that B1D contains LH, and B3D and B5D contain FSH-like gonadotropin. SDS PAGE analysis in combination with immunoblot revealed that B1D was almost pure LH, but B3D seemed to be not homogeneous. Anti-B1D-serum and anti-B3D-serum were raised in rabbits, and radioimmunoassays (RIAs) for B1D and B3D were established. The cross reactivity of B3D and B5D in BID-RIA was about 30% of B1D, while that of B1D in B3D-RIA was only 3% of B3D and B5D. These RIAs were sensitive enough to measure gonadotropins in plasma samples of Bufo japonicus.

• 社団法人日本動物学会の論文
• 1989-10-15

著者

• Itoh M

  Primate Research Institute Kyoto University

• Itoh Mariko

  農林水産省農業生物資源研究所

• TAKADA Koji

  Department of Biochemistry [I], Jikei University School of Medicine

• ITOH Masanori

  Department of Chemistry, St. Marianna University School of Medicine

• NISHIO Hiroshi

  Department of Clinical Pathology, Kinki University School of Medicine

• ISHI SUSUMU

  Department of Biology, School of Education, Waseda University

• Ishii S

  Department Of Biological Science Graduate School Of Science Tokyo Metropolitan University

• Itoh Masanori

  Department Of Chemistry St. Marianna University School Of Medicine

• Nishio H

  Waseda Univ. Tokyo Jpn

• Nishio Hiroshi

  Department Of Biology School Of Education Waseda University

• Takada K

  Soka Univ. Tokyo Jpn

• Takada Koji

  Department Of Applied Electronics Tokyo Institute Of Technology

• Itoh Masanori

  Department Of Chemistry

• Itoh Masanori

  Department Of Biology College Of Liberal Arts And Sciences Tokyo Medical And Dental University

• ISHI SUSUMU

  Department of Biological Science, Graduate School of Science, Tokyo Metropolitan University

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