DEMONSTRATION OF THE LOCALIZATION OF MUSCARINIC ACETYLCHOLINE RECEPTORS IN THE GASTRIC MUCOSA : LIGHT AND ELECTRON MICROSCOPIC AUTORADIOGRAPHIC STUDIES USING ^3H-QUINUCLIDINYL BENZILATE

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概要

• 論文の詳細を見る

• For autoradiographic demonstration of the muscarinic acetylcholine receptor (m-AChR) in the gastric mucosa, ^3H-quinuclidinyl benzilate (^3H-QNB), a potent muscarinic antagonist, was administered to rats at a constant rate of 1.4 ml per hr via an aortic catheter. Fixation of the tissue was made by a freeze-drying procedure, followed by the wet-sectioning of the Epon blocks with ethylene glycol and the dry-mounting of the emulsion to obliterate loss of the water soluble radiolabeled antagonist. To examine the specificity of the autoradiographic reaction, the atropine-treated and the vagotomized rats were also treated in the same manner as the control rats. As a result, it has been confirmed that the parietal cell among the gastric epithelial cells is a major site of labeling of ^3H-QNB, whereas few grains are located over the surface epithelium. Some of the grains were also closely associated with the true capillary endothelium in the gastric mucosa. Electron microscopic autoradiography revealed that most of the grains were in close proximity to either the plasma membrane of the parietal cell or the basal plasma membrane of the true capillary endothelium. Preadministration of atropine resulted in a decrease of the labeling of ^3H-QNB, whereas the vagotomy caused an increase in the labeling. The above-findings strongly support our hypothesis previously proposed on the basis of the localization of the acetylcholinesterase, that the parasympathetic nerve would have some direct effects on the true capillary as well as on the parietal cell. In addition, the m-AChR was found to be dispersed both on the true capillary endothelium and on the parietal cell plasma membrane by electron microscopic autoradiography, compared with the nicotinic AChR in the neuromuscular junction.

• 日本組織細胞化学会の論文

著者

• KOMATSU Hirokazu

  Department of Medical Oncology and Immunology, Nagoya City University Graduate School of Medical Sci

• Komatsu H

  Keio Univ. Tokyo Jpn

• TSUKADA Nobuhiro

  Department of Hematology/Oncology, The Institute of Medical Science, The University of Tokyo

• YONEI Yoshikazu

  Department of Internal Medicine, Nihon Kokan Hospital

• ODA Masaya

  Department of Internal Medicine School of Medicine, Keio University

• Watanabe Norihito

  Department Of Internal Medicine Tokai University School Of Medicine

• Watanabe Norihito

  Department Of Internal Medicine School Of Medicine Keio University

• Oda M

  Organized Center Of Clinical Medicine International University Of Health And Welfare

• Oda Masaya

  Department Of Gastrointestinal Medicine School Of Medicine Keio University

• Ogi Mariko

  Laboratory Of Pathology Kitasato Institute Medical Center Hospital

• NAKAMURA Masahiko

  Deparment of Physics,Faculty of Science,Kyoto University

• TSUCHIYA Masaharu

  Department of Internal Medicine, School of Medicine, Keio University

• Komatsu Hirokazu

  Department Of Applied Chemistry Keio University

• Tsukada Nobuhiro

  Department Of Hematology/oncology The Institute Of Medical Science The University Of Tokyo

• Tsukada Nobuhiro

  Department Of Internal Medicine Saiseikai Central Hospital

• Yonei Y

  Department Of Internal Medicine Nihon Kokan Hospital

• Yonei Yoshikazu

  Department Of Internal Medicine Nippon Kokan Hospital

• 中村 正彦

  Departments Of Radiology And Biomedical Engineering Tokai University Medical School

• Nakamura Masahiko

  Department Of Physiology Saitama Medical School

• Oda Mizue

  Organized Center Of Clinical Medicine International University Of Health And Welfare

• Yonei Y

  Department Of Internal Medicine Nippon Kokan Hospital

• Tsuchiya Masaharu

  Department Of Internal Medicine Keio University

• Nomura Masahiko

  Department Of Physiology Saitama Medical School

• Tsukada Nobuhiro

  Department of Hematology and Oncology, Institute of Medical Science University of Tokyo

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