サケ科魚類稚魚の内臓真菌症に関する研究-2-アマゴ稚魚の腹腔内より分離された真菌の性状

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An epizootic of visceral mycosis occurred among hatchery-reared fry of Gifu Prefecture (TASHIRO et al., 1977). Two kinds of fungi were isolated from diseased amago salmon fry by inoculating materials from the abdominal cavity onto Sabouraud dextrose agar and incubating at 20°C.<BR>One kind of fungus, strain GFA 7501 was identified as Saprolegnia diclina according to SEYMOUR (1970) from its characteristics of the asexual reproductive stages and the sexual reproductive structures. Histopathological observations suggested that the primary site of infection of this fungus might be the pars pylorica of stomach.<BR>Another strain GFA 7502 could not be identified because evident sporulation was observed neither on Sabouraud dextrose agar nor on agar block in water culture. It had filamentous mycelia with septa. The hyphae was 4 to 12μ in diameter (Fig. 7). Spherical structures formed on hyphae after a prolonged period of time were usually 14 to 20μ in diameter. The character of the structures was unknown. Histopathological observations suggested that the primary site of infection of strain GFA 7502 which was named tentatively as an unidentified filamentous fungus with septa might be the pars cardiaca of stomach or the air bladder.<BR>Histopathological observations were made on 7 diseased amago salmon fry. Of the 7 fry, 5 were found to be infected with only S. diclina, while 2 fry showed a complicated infection of S. diclina and an unidentified filamentous fungus with septa.<BR>Effects of temperature, pH, malachite green and methylene blue on the growth of hyphae of the strain GFA 7501 and the strain GFA 7502 were also described in this paper (Table 2).
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サケ科魚類稚魚の内臓真菌症に関する研究-2-アマゴ稚魚の腹腔内より分離された真菌の性状

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