ドジョウから分離された病原性粘液細菌Chondrococcus columnarisの性状

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A Chondrococcus-like bacterium of high virulence was isolated from diseased loaches (Misgurnus anguillicaudatus). The basal medium used in this study as the standard for various purposes contains 0.3 per cent Bacto-tryptone and 0.2 per cent yeast extract, adjusted to pH 7.5 (TY broth). TY agar media containing 1.5 per cent agar and 0.4 per cent agar were suitable for the isolation and the maintenance of the bacterium, respectively. Stock cultures were stored in a refrigerator and subculture was made every two or three weeks. Colonies on TY agar plates were usually yellow and flat and have irregular, rhizoidar edges, though showing a wide variation in form in older cultures. (Fig. 2). Vegetative cells from lesions or young cultures were Gram-negative, slender, flexible rods and have 3 to 8 microns in length and about 0.5 microns in width. Under cultural conditions the cells usually went through degenerative processes similar to those described by GARNJOBST3) and by BORG4). A swinging motion and column formation were observed in materials scraped directly from lesions and placed in a wet mount. (Fig. 3). A sliding movement was also observed on solid media. The formation of fruiting bodies and microcysts was clearly demonstrated by using BORGs method. (Fig. 5). Tests for biochemical characteristics of the bacterium yielded the results shown in Table 2. On the basis of the information obtained, the organism was identified with Chondrococcus columnaris (DAVIS) ORDAL and RUCKER (1944).
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ドジョウから分離された病原性粘液細菌Chondrococcus columnarisの性状

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