ヒラメ鰭由来の一細胞系〔英文〕

概要

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A cell line, designated as FF-11, was developed from Japanese flounder (Paralichthys olivaceus) fin. This epithelioid cell has been passed 45 times in a period of 2 years in Leibovitz's L-15 medium supplemented with 20% fetal bovine serum (FBS) at 20deg;C. A modal number of chromosomes at passage 42 was 48, which is the same as that of normal flounder somatic cells. The temperature range from 20 to 28deg;C supported the growth of FF-11 cell and optimal growth occurred at 25 to 28deg;C, but 37deg;C was lethal. Cells were able to be stored at a -80deg;C with 10% dimethyl sulphoxide for at least 12 months and no mycoplasmal, bacterial or fungal contaminations were detected. The FF-11 cell line was susceptible to yellowtail ascites virus (YAV) and Rhabdovirus olivaceus (HRV), but the susceptibility was lower than those of established fish cell lines (FHM, EPC, CHSE-214, RTG-2). It was tried to isolate flounder herpesvirus (FHV), the causative agent of epidermal hyperplasia of flounder larvae, by FF-11 cell, but to no avail.
日本魚病学会の論文