炭化水素を炭素源とする酵母の生産に関する研究-2-培養条件の検討-1-

概要

論文の詳細を見る
The effects of various nitrogen sources and surfactants on the growth of hydrocarbon utilizing yeast strain, Candida sp. Y 191, which had been isolated from oily soil by M. Akaki, one of the authors, were investigated in shaking culture on the basal medium B containing I per cent cetane as carbon source. The results obtained were as follows. Four kinds of nitrogen source, i.e., urea, ammonium sulfate, ammonium chloride and ammonium nitrate, were tested. Among these four,urea was most fitted for the growth of the yeast, and gave higher yeast cell yield than the other nitrogen sources tested. Optimal concentration of urea as a nutrient for the yeast growth in the medium containing 1 per cerit cetane as carbon source was between 0.2 and 0.8 per cent. Ten kinds of surfactant shown in Table 3 were tested. Among them, Span 40, Span 80, Span 85 gave good effects on the growth of the yeast in the basal medium. Especially, Span 85 was most effective for the yeast growth. Optimal concentration of Span 85 for the growth of the yeast in the medium was between 0.005 and 0.01 per cent. The yeast cell yield on the basis of a definite amount of the medium decreased with the increase of the volume of medium in a culture flask. However, the addition of Span 85 to the basal medium greatly prevented the yeast cell yield on the basis of a definite amount of the medium from decreasing.
三重大学農学部の論文