ステビアの組織培養と成分に関する研究(第1報)

概要

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Callus was induced from stem segments and shoot tips of Stevia rebaudiana BERTONI on Murashige and Skoog (MS) medium containing 2,4-D(10^<-7>-10^<-5> M) and kinetin (0-10^<-5> M) in the dark or light. The growth rate of callus culture increased as the concentration of 2,4-D in the medium was increased. Differentiation into shoot and root was observed during the cultivation of callus. In the case of shoot tips, the most favourable condition was found to be cultivation on MS medium containing 10^<-5> M kinetin and 10^<-7> M NAA in the light for shoot formation and cultivation on MS medium containing 10^<-5> M NAA under irradiation for the growth of adventitious root. In the case of stem segments, shoot formation was not observed on MS medium, but adventitious embryoid formation was achieved on a series of 10^<-5> M 2,4-D containing medium. The plantlets were successfully transplanted to sterilized soil. Thin layer chromatography showed the presence of stevioside and rebaudioside A in MeOH extract from shoot, but those compounds were not detected in the extract from callus culture. Some callus cultures were found to contain another glycoside when incubated under irradiation.
日本生薬学会の論文
1984-03-20