茎頂培養によるミシマサイコの増殖

概要

論文の詳細を見る
Shoot tips of Bupleurum falcatum L. (Umbelliferae) were cultured in 27×200 mm culture vials containing liquid medium with slow shaking at 2 rpm on a gyrated drum placed obliquely. Somatic embryos were induced in Murashige-Skoog (MS) liquid medium containing 10^<-5>M BAP or in Gamborg B5 liquid medium containing 10^<-6>M BAP and 0, 10^<-7>, 10^<-6>M NAA at 25±2℃ under continuous illumination. The somatic embryos derived in MS medium contained white callus, but those obtained in B5 medium did not. The somatic embryos proliferated in liquid MS medium supplemented with 10^<-5>M BAP at a rotation speed of 95 rpm, at 25±2℃, under continuous illumination while direct plantlet formation from the matured embryos occurred in the B5 liquid medium during 10 weeks. The embryos germinated on the hormone free MS or B5 gelrite medium to give plantlets with the phenotype identical to the mother plant's phenotype.
日本生薬学会の論文
1987-12-20