二波長薄層クロマトグラフデンシトメトリーによるサイコサポニンの分離定量とその栽培ミシマサイコの品質評価への応用

概要

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A simple and quick quantitative analysis of bioactive principles of Bupleuri radix, i.e. saiko-saponins-a(1) and -d(3) has now been established. When methanolic extracts of the crude drug were treated with acid, (1) and (3) contained therein were quantitatively converted into corre-sponding dienesaponins, saikosaponins -b_1(6) and -b_2(7), respectively which have absorption maxima at around 240 run. Dual wave length densitometry of a thin layer chromatogram of the acid-treated extracts at 240 nm (λ_S) and 350 nm (λ_R), clearly showed the location and the quantity of each saponins on the plate. This procedure was applied to pharmaceutical evaluation of the root of Bupleurum falcatum L. cultivated at Izu Experimental Station of Medicinal Plants, National Institute of Hygienic Sciences.
1980-12-20