20914 生体内環境を考慮した抗血栓性評価(OS9 バイオエンジニアリング(3),オーガナイズドセッション)

概要

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Research on the hemocompatibility of materials is required for the development of artifical organs, which contact with blood. However assessing haemocompatibility in vitro is difficalt.Shear-induced platelet aggregation (SIPA) is an important factor in thrombus formation under share flow in a blood vessel. A cone and plate type viscometer was used to simulate in vivo blow. In this experiment, platelet adhesion on materials under flow conditions was tested with shar rates of 1, 50, 150s^<-1>. Activation occurs after platelets adhere to a surface, which leads to thrombosid. The elevation in cytosolic Ca concentrations was used as a marker of activation. Result showed changes in platelet cytosolic Ca^<2+> concentration during real-time platelet adhesion on material surface under flow conditions. Reconstituded blood contain platelets treated with 2-fluorochrome, which has a different fluorescence wavelength from the excitation wavelength. We use W-view system and an epifluorescence microscope for real time imaging.
一般社団法人日本機械学会の論文
2010-03-09