1009 細胞膜微小変形が惹起する複数細胞内シグナルの同時観察(S03-2 細胞・生体分子のバイオメカニクス(2),S03 細胞・生体分子のバイオメカニクス)

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To study signal transduction induced by mechanical stress, inhibition experiments, which inhibit the function in specific or broad range of proteins, are often used. Although inhibition is a relatively simple procedure, it has some limitations such as inhibition specificity and lacking of understanding spatial-temporal distribution of proteins. To study intracellular Ca^<2+> transient cascade evoked in plasma membrane micro-deformed Mardin-Darby Canine Kidney Cell, we dual-imaged PIP_2 hydrolysis and Ca^<2+> transient with PLCδ1PH-CFP/PLCδ1PH-YFP Fura-2 respectively. Our results indicate intracellular Ca^<2+> transient evoked by plasma membrane micro-deformation involve, at least in part, the mechanism that is independent of stretch-activated Ca^<2+> channel.
社団法人日本機械学会の論文
2007-09-07