1247 周期的引張刺激に応答する細胞形態変化の単一細胞レベルでの可視化(G02-7 細胞,G02 バイオエンジニアリング)

概要

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Endothelial cells are known to change their shapes aligned perpendicular to the direction of cyclic stretch. However, the mechanisms are still not clear. In this study, we developed a new cell-stretching device which allows continuous observation of cyclically stretched/relaxed cells. By using this apparatus, morphological changes of single cells in response to cyclic stretch (1Hz, 10%, 2 hours) were visualized and analyzed. The data showed that there were two major patterns of morphological changes of ECs, depending on their initial shapes. While ECs with round shapes at initial state changed their shapes to become more elongated in a direction perpendicular to the stretch, cells oriented and elongated in the stretch direction at initial state changed their shapes to become rounder first and then to become elongated perpendicular to the stretch direction. In addition, this cyclic-stretch-induced endothelial morphological change was blocked by Y-27632, a Rho-related kinase inhibitor, or blebbistatin, a myosin inhibitor. Taken together, cell retraction as well as elongation is important in the stretch-induced endothelial morphological changes, and this contraction may be induced by stress fiber contraction via ROCK-mediated myosin phosphorylation.
2005-09-18