217102 共焦点顕微鏡とImageJによる再生軟骨の階層性評価(OS07 細胞・生体組織1,オーガナイズド・セッション)

概要

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Many cartilage tissue engineering studies use mechanical forces to enhance the biomechanical properties of the engineered tissue. Then it is measured mainly as overall property. But, partial property is also important, because cartilage has configurationality. So, this paper describes a new estimation of regenerated cartilage configurationality by confocal microscope and ImageJ. First, three-dimensional image of cartilage is obtained by confocal microscope. Second, the image is analyzed by imageJ and it outputs volume, surface fraction, intensity and position of the chondrocytes. This study applies this method to native cartilage, cultured cartilage, static cultured cartilage and rotating cultured cartilage. The result shows that this method captures cartilage configurationality.
一般社団法人日本機械学会の論文
2011-03-17