69 ヤマブシタケ(Hericium erinaceum)の産生する生物活性物質(ポスター発表の部)

概要

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In the course of our continuing research aimed at the isolation of biological active compounds from mushrooms, we found nine new compounds in an edible mushroom Hericium erinaceum. Fresh fruiting bodies of H. erinaceum were extracted with acetone, and the extract was concentrated and fractionated by solvent partitions (CHCl_3 then ethyl acetate). Repeated column chromatography (SiO_2) followed by HPLC and/or recrystallization of the CHCl_3 extract gave eight new benzene-derivatives hericenones (3-5, 7-9) and a novel fatty acid(10). Hericenone A(1), B(2) and the fatty acid(10) were cytotoxic against HeLa cells; minimum concentration giving complete death of the cells for 1 was 100μg/ml, for 2 was 6.3μg/ml, and for 10 was 100μg/ml, respectively. Hericenones C(3)-E(5) and F(7)-H(9) were not toxic toward the cells, but exhibited stimulating activity of nerve growth factor (NGF)-synthesis in vitro; in the presence of 3, 4 and 5 at 33μg/ml, mouse astroglial cells secreted 10.8±0.8, 23.5±1.0 and 13.9±2.1pg/ml NGF into the culture medium, respectively. 7-9 exhibited similar level of the activity. 3-5 and 7-9 are first active compounds isolated from other than animals. On the other hand, 1 and 2 did not have the stimulating activity. 3-5 and 7-9 have also inhibitory activity of prostaglandin E_2 production in vitro.
1991-09-07