70(P-27) カキシメジ(Tricholoma ustale)の毒について(ポスター発表の部)

概要

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Tricholma usutale (Japanese name: kakishimeji) is one of notorious poisonous mushrooms. However, the toxic principle in the mushroom had been unknown. We describe the isolation, structure determination of the toxin from the mushroom. Bioassay was done as follow. After direct administration of a compound (or a fraction) into mice's stomach by using catheters, their behavior and symptoms were observed. Fruiting bodies of Tricholma usutale were extracted with 85% ethanol. The extract was partitioned between chloroform and water, and then ethyl acetate and water. The chloroform soluble fraction was chromatographed on a silica gel column followed by HPLC using ODS column, giving compound 1. FAB-MS (positive; matrix NOBA) of compound 1 showed the [M+H]^+ ion at m/z 339, [M+Na]^+ ion at m/z 361. ^1H-NMR showed a methylene group and aromatic groups (ten protons). ^<13>C-NMR showed eight signal of δ96.4, 115.1, 128.1, 128.4, 130.0, 133.5, 149.4 and 174.4. Finally, the structure of compound 1 was determined by the X-ray crystallographic analysis. Compound 1 caused the decrease of activity of the mice and finally the mice died.
2001-09-01