34 担子菌が生産する配糖体抗生物質Glykeninの構造研究(ポスター発表の部)

概要

論文の詳細を見る
A strain of Basidiomycetes sp. has been to produce glycosidic antibiotics glykenin (GK), which exhibit inhibitory activity against gram-positive bacteria. GK complex are composed of two major components (GK-III and IV) and five minor components (GK-I, II, V〜VII) on the silica gel TLC. Alkaline hydrolysis of GK complex gave deacetyl derivative DG-A〜C, which are basic structures of GK. DG-A〜C are composed of three unusual hydroxylated C_<26> long-chain fatty acids (1a〜c) as aglycones and trisaccharide, respectively. The location of the trisaccharide chain was determined by permethylation, methanolysis and PCC oxidation of aglycone 1a〜c The HPLC analyses of peracetyl phenacyl esters of GK-II〜IV and VI, showed three peaks which were coincided with those of the peracetyl phenacyl esters of DG-A〜C. Major components GK-III and IV, separated by repeated silica gel chromatography, have molecular weight 970 that was indicated the presence of two acetyl groups on the structures of DG-A〜C. The positions of two acetyl groups in GK-III and IV were determined by the fragmentation analysis of negative SIMS spectra and by DQF ^1H-COSY, Relayed COSY and 2-Relayed COSY techniques and their structures were determined as shown in Fig. 5.
天然有機化合物討論会の論文
1990-09-25