717 ピラー型ナノ構造配列を利用した電気泳動DNA分離における駆動電圧とピラー形状の影響(S07-3 マイクロ・ナノバイオメカニクス(3),S07 マイクロ・ナノバイオメカニクス)

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We have demonstrated effective biomolecule separation methods based on size exclusion chromatography (SEC) type separation using nanostructured channels constituted with a flat pillar matrix and a convex pilar matrix. The performances of biomolecule separations were compared between different applied voltages and between the flat and convex pillar matrices. In the SEC type separation using the flat pillar matrix, the narrow lanes were designed for the passages for smaller molecules to detour. The specific feature of the convex fence matrix is that the lane width is changed at the halfway of the lane length. It was found from the result of the separation of λ-Hin d III digest (125-23130bp) that the separation resolution of the long DNA was highest for the lowest voltage of 20V. In contrast, the result of the separation of φX 174-Hae III digest (72-1353bp) showed that the separation resolution of the short DNA was highest for the highest voltage of 100V. In addition, it is interesting to note that the convex fence matrix enabled us to separate the peaks of 4, 6 and 9kbp fragments from the peak of 23kbp, while those peaks overlapped each other for the flat fence matrix.
2006-09-15