フェンス状ナノ構造配列を利用した電気泳動に基づくDNA分離に及ぼす印加電圧とフェンス形状の影響(機械力学,計測,自動制御)

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We have demonstrated effective biomolecule separation methods based on size exclusion chromatography (SEC) type separation using nanostructured channels constituted with a flat fence matrix and a convex fence matrix. The performances of biomolecule separations were compared between different applied voltages and between the flat and convex fence matrices. The flat fence matrix has wide streets along the electrophoresis direction and narrow lanes perpendicular to the streets. In the SEC type separation using the flat fence matrix, the narrow lanes were designed for the passages for smaller molecules to detour. The specific feature of the convex fence matrix is that the lane width is changed at the halfway of the lane length. It was found from the result of the separation of λ-Hin d III digest (125-23 130 bp) that the separation resolution of the long DNA was highest for the lowest voltage of 20V. In contrast, the result of the separation of φX 174-Hae III digest (72-1353 bp) showed that the separation resolution of the short DNA was highest for the highest voltage of 100V. In addition, it is interesting to note that the convex fence matrix enabled us to separate the peaks of 4, 6 and 9 kbp fragments from the peak of 23 kbp, while those peaks overlapped each other for the flat fence matrix.
2007-02-25