血液脳関門について : I.中枢神経系における6-aminonicotinamide中毒下の螢光色素(Evans blue)の分布

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A study was made on the toxic effect of 6-AN on the vascular permeability in the rat brain and spinal cord. 120 rats were examined by light and fluorescence microscopies, 4-24 hours following intraperitoneal application of 6-AN (8, 10, 20, 50mg/kg). Evans blue (1ml, 2%) was given intravenously as the tracer 2 hours before the sacrifice. Brain stem and grey matter of the spinal cord revealed injury to the blood-brain barrier (BBB) according to the microscopic lesions. Higher doses increased the intensity and shortened the latency of penetration of Evans blue into the involved structures, showing a characteristic peak for each dosage, and fading away 22-24 hours after application of 6-AN. Microcirculatory disturbances probably inhibited further penetration of Evans blue into the affected structures. The structures of the spinal cord and brains tem showing the red fluorescence of Evans blue varied depending on the fixation method. After perfusion fixation (Bouin solution or formaldehyde : pH 7, 4), the blood vessel wall, the neuropil and nuclei of necrotic glial cells showed fluorescence. The cytoplasm of injured glial cells and neurons failed to demonstrate fluorescence. After immersion fixation (Bouin solution), Evans blue-tracing was seen mainly in shrunk and pycnotic nuclei. The comparison of Evans blue-fluorescence after perfusion and immersion fixation gives evidence that the staining pattern in tissue fixed by immersion reflects a supravital respective postmortal artefact. The experiments suggest that Evans blue enters the brain tissue via the extracellular space, then staining mainly damaged structures of the neuropil, the glial cells and neuronal pericarya.
帯広畜産大学の論文
1976-06-25

血液脳関門について : I.中枢神経系における6-aminonicotinamide中毒下の螢光色素(Evans blue)の分布

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