導電性中空糸膜を利用したウイルス遺伝子検査法における血清間個体差の影響およびRNAウイルスを対象とした検査への応用に関する検討

概要

論文の詳細を見る
The Japanese Red Cross Society has been employing the nucleic acid amplification testing (NAT) to secure the microbiological safety of blood products. The serum has individual difference because the blood is collected from many humans located in various parts of the country. The association reported that the results of NAT were influenced by slight differences in blood components. We designed a viral detection method consisting of a metal-coated hollow fiber (MCHF)-membrane and loop-mediated isothermal amplification (LAMP). This detection method was highly sensitive and much quicker than NAT. In this study, we used two kinds of simulated serum samples that used the bovine serums of different lot numbers and reviewed stability for slight differences in blood components in terms of the designed viral gene detection. And we reported the fundamental examination of feline calicivirus to design RNA virus detection method metal-coated hollow fiber-membrane and gene amplification.We examined the influence of individual differences on only LAMP for bovine serum of different lot numbers as the first step. LAMP couldn't detect the virus in nay of the simulated serum samples. As the next step, we tested the influence of serum individual differences on viral detection system consisting of MCHF-membrane and LAMP. As a result, this viral detection method was able to detect herpes simplex virus type 1 from simulated serum samples containing more than 10 PFU/mL of virus without influence from individual differences. This viral detection method is stable and not influenced by serum individual differences, and is thus an epoch-making method. For application in the clinical spot, we established reverse transcriptase-polymerase chain reaction (RT PCR) using feline calicivirus. We could acquire fundamental knowledge for RNA virus detection method consisting of a metal-coated hollow fiber-membrane and RT-PCR.
2007-03-26

導電性中空糸膜を利用したウイルス遺伝子検査法における血清間個体差の影響およびRNAウイルスを対象とした検査への応用に関する検討

スポンサーリンク

概要

著者

関連論文

スポンサーリンク