Glycyrrhizin β-D-Glucuronidase of Eubacterium sp. from Human Intestinal Flora(Biological)

概要

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A bacterial strain capable of hydrolyzing glycyrrhizin (GL) to glycyrrhetic acid (GA) was isolated from human feces. This bacterium was identified as Eubacterium sp. The GL-hydrolyzing activity increased in parallel with the growth of this bacterium, which also produced β-D-glucuronidase (EC 3.2.1.31) acting on β-D-glucuronides of phenolic compounds such as phenol-phthalein mono-β-D-glucuronide. GL-hydrolyzing activity was recovered in the supernatant fraction after disruption of this bacterium with a French press and was partially purified by means of ammonium sulfate fractionation, and Sephadex G-200 and octyl-Sepharose column chroma-tographies. GL-hydrolyzjng enzyme was separated from the β-D-glucuronidase which hydrolyzes β-D-glucuronides of phenolic compounds by octyl-Sepharose column chromatography, indicating that the GL-hydrolyzing enzyme is a novel type of β-D-glucuronidase.
公益社団法人日本薬学会の論文
1987-02-25