816 表面形状をナノスケールで制御した材料上における細胞の接着挙動観察(GS-2 生体材料,研究発表講演)

概要

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Bio-environment designing is the key factor for successful tissue engineering technology. In this study, the relationship between the cell behavior and the surface morphology, which is controlled by dynamic oblique deposition, has been discussed with a special emphasis on the shapes and actin filament synthesis. The SiO_2 films with flat, circular and elongated surface morphologies were deposited on a glass substrate. The aspect ratio of circular morphology was about 1.2 and spacing between each cluster of column is about 100nm. The aspect ratio and spacing of elongated morphology was about 2.2 and 50-100nm, respectively. Mesenchymal cells derived from bone marrow were seeded on the each sample and then adhesion behaviour, shape of cell and actin filament were observed. Adhesion rate of cells on the flat surface sample were higher than that on the others. Observation of actin filament showed that cells on the elongated surface revealed much actin filament per unit cell adhesion area and had higher aspect ratio than on the circular. Some kind of cell function might be affected by the nano morphologies of the surface. There is the possibility that cell shape and structure can be controlled by nano morphologies of surface.
社団法人日本機械学会の論文
2005-03-18