Distinct Glycosylation in Interstitial and Serum Tenascin-X(Molecular and Cell Biology)
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概要
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We developed an easy and fast method to isolate extracellular matrix tenascin-X (TNX) from various tissues in mice based on TNX antibody affinity purification. We purified approximately 350-kDa cellular interstitial TNX (iTNX) from the spleen, liver and kidney as well as 200-kDa serum TNX (sTNX). Since the nature and significance of glycosylation in TNX remains to be elucidated, glycobiochemical properties of purified TNX were characterized by lectin blot analysis. Lectin blots by Con A, LCA, PHA-E4, RCA120 or WGA revealed the presence of N-glycan in the cellular TNX and especially complex-type N-glycan in the serum TNX. In addition, the iTNX from liver and kidney also possessed O-glycan based on the reaction to PNA. The binding to AAL indicated that iTNX from the three tissues possesses fucose linked α1,6 to a pentasaccharide core, whereas sTNX does not. The reaction to SSA but not to MAM suggested the presence of sialic acid linked α2,6 to galactose in both cellular and serum TNX. Lectin blots of trypsin-treated iTNX from the spleen also demonstrated that WGA alone reacts to the t300 product derived from the amino-terminal 300-kDa portion.
- 公益社団法人日本薬学会の論文
- 2007-02-01
著者
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Matsumoto Ken-ichi
Department of Biosignaling and Radioisotope Experiment, Center for Integrated Research in Science, S
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Matsumoto Ken-ichi
Dep. Of Biosignaling And Radioisotope Experiment Center For Integrated Res. In Sci. Shimane Univ.
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Matsumoto Ken-ichiro
Departments Of Physical Chemistry Showa Pharmaceutical University
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ARIGA Hiroyoshi
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University
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Ariga Hiroyoshi
Department Of Molecular Biology Graduate School Of Pharmaceutical Sciences Hokkaido University
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MATSUMOTO Ken-ichi
Denartment of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University
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KINOSHITA Takeshi
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University
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Matsumoto Ken-ichi
Department Of Molecular Biology Graduate School Of Pharmaceutical Sciences Hokkaido University
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Matsumoto Ken-ichi
Department Of Bioresource Science Faculty Of Agriculture Shimane University
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Kinoshita Takeshi
Department Of Material Science Faculty Of Science And Technology Keio University
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Kinoshita Takeshi
Department Of Molecular Biology Graduate School Of Pharmaceutical Sciences Hokkaido University
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Ariga H
Graduate School Of Pharmaceutical Sciences Hokkaido University
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Ariga Hiroyoshi
Deparnment Of Molecular Biology Graduate School Of Pharmaceutical Sciences Hokkaido University
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Kinoshita Takeshi
Department of Applied Chemistry, Faculty of Engineering, Keio University
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