Tenascin-X Induces Cell Detachment through p38 Mitogen-Activated Protein Kinase Activation
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概要
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Extracellular matrix glycoprotein tenascin-X (TNX) is the largest member of the tenascin family. In this study, we investigated the adhesive properties of TNX and the signaling pathway to be induced to mouse fibroblast L cells on TNX substrate. Approximately 45% of evaluable cells used in the cell adhesion assay were attached to purified TNX but did not spread and were rounded on TNX. The remaining 55% of cells were detached from the TNX substrate and were floating in the conditioned medium. In rounded cells on TNX, phosphorylation of focal adhesion kinase (FAK) was diminished compared with that in cells on control phosphate buffered saline (PBS). To better understand the pathways that lead to the detachment of cells on the TNX substrate, we examined phosphorylation of p38 mitogen-activated protein (MAP) kinase. Phosphorylation of p38 MAP kinase was observed in the rounded cells on TNX in a dose-dependent manner, and the maximum effect was observed at 30 min on TNX. Inhibition of p38 MAP kinase α expression by RNA interference partially suppressed the TNX-induced cell detachment. These results suggest that the p38 MAP kinase is a major mediator of TNX-induced cell detachment.
- 公益社団法人 日本薬学会の論文
著者
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Matsumoto Ken-ichi
Department of Biosignaling and Radioisotope Experiment, Center for Integrated Research in Science, S
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ARIGA Hiroyoshi
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University
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MAITA Hiroshi
Department of Mechanical Systems and Design, Tohoku University
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Maita Hiroshi
Department Of Mechanical Systems And Design Tohoku University
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Ariga Hiroyoshi
Department Of Molecular Biology Graduate School Of Pharmaceutical Sciences Hokkaido University
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Fujie Shinpei
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University
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Matsumoto Ken-ichi
Department Of Bioresource Science Faculty Of Agriculture Shimane University
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Ariga Hiroyoshi
Deparnment Of Molecular Biology Graduate School Of Pharmaceutical Sciences Hokkaido University
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Maita Hiroshi
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University
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