In Vitro Metabolism of (-)-Camphor Using Human Liver Microsomes and CYP2A6(Biochemistry)

スポンサーリンク

概要

• 論文の詳細を見る

• The in vitro metabolism of (-)-camphor was examined in human liver microsomes and recombinant enzymes. Biotransformation of (-)-camphor was investigated by gas chromatography-mass spectrometry (GC-MS). (-)-Camphor was oxidized to 5-exo-hydroxyfenchone by human liver microsomal cytochrome (P450) enzymes. The formation of metabolites of (-)-camphor was determined by the relative abundance of mass fragments and retention time on gas chromatography (GC). CYP2A6 was the major enzyme involved in the hydroxylation of (-)-camphor by human liver microsomes, based on the following lines of evidence. First, of eleven recombinant human P450 enzymes tested, CYP2A6 catalyzed the oxidation of (-)-camphor. Second, oxidation of (-)-camphor was inhibited by (+)-menthofuran and anti-CYP2A6 antibody. Finally, there was a good correlation between CYP2A6 contents and (-)-camphor hydroxylation activities in liver microsomes of 9 human samples.

• 公益社団法人日本薬学会の論文
• 2007-02-01

著者

• Miyazawa Mitsuo

  Department Of Aplied Chemistry Faculty Of Science And Engineering Kinki University

• GYOUBU Kunihiko

  Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University

• Gyoubu Kunihiko

  Department Of Applied Chemistry Faculty Of Science And Engineering Kinki University

関連論文

• Inhibition of Helicobacter pylori Motility by (+)-Syringaresinol from Unripe Japanese Apricot(Medicinal Chemistry)
• Endoscopic pancreatic duct drainage and stenting for acute pancreatitis and pancreatic cyst and abscess
• Metabolism of (+)- and (-)-Menthols by CYP2A6 in Human Liver Microsomes
• Volatile Components of the Straw of Oryza sativa L.
• Comparison of Larvicidal, Adulticidal and Acaricidal Activity of Two Geometrical Butylidenephthalide Isomers(Pharmacognosy)
• Biotransformation of (-)-Menthone by Human Liver Microsomes
• Volatile Flavor Components of ZINGIBERIS RHIZOMA (Zingiber officinale Roscoe)(Organic Chemistry)
• 炭疽病菌による4-propylcyclohexanoneおよび4-isopropylcyclohexanoneの立体選択的還元
• 炭疽病菌による4-tert-butylcyclohexanoneの立体選択的還元
• The Constituents of the Essential Oil from Erigeron philadelphicus
• Convenient Synthesis of Arylmethylenesuccinic Acids via Wittig Reaction
• Character Impact Odorants of Wild Edible Plant-Cacalia hastata L. var. orientalis-Used in Japanese Traditional Food
• Hepatic Metastasis 12 Years after Nephrectomy for Renal Cell Carcinoma
• Inhibitory Compound of Tyrosinase Activity from the Sprout of Polygonum hydropiper L.(Benitade)(Pharmacology)
• Regioselective Oxidation of (+)-α-Longipinene by Aspergillus niger
• Cyclooxygenase-2 inhibitory effects and composition of the volatile oil from the dried roots of Lithospermum erythrorhizon
• Constituents of Essential Oil from the Dried Fruits and Stems of Akebia quinata (THUNB.) DECNE.
• Suppression of the SOS-Inducing Activity of Trp-P-1 and Aflatoxin B_1 by Meso-dihydroguaiaretic Acid from Machilus thunbergii in the Salmonella typhimurium TA1535/pSK1002 umu Test
• Biotransformation of Sinesetin by the Larvae of the Common Cutworm (Spodoptera litura)(Biochemistry/Molecular Biology)
• α-Glucosidase Inhibitor from Bergenia ligulata
• Antimutagenic Compound from Yellow batai (Peltophorum dasyrachis)
• Components of Essential Oil from Woods of Prunus mume Sieb. et Zucc.
• Antimutagenic Activity of Isoflavone from Belamcanda chinensis
• Antimutagenic Activity of Gigantol from Dendrobium nobile
• Antimutagenic Activity of Falcarindiol from Peucedanum praeruptorum
• Antimutagenic Activity of (+)-β-Eudesmol and Paeonol from Dioscorea japonica
• Antimutagenic Activity of Flavonoids from Chrysanthemum morifolium(Biochemistry & Molecular Biology)
• Suppression of Chemical Mutagens-Induced SOS Response by Phenolic Acids from Black Rice Bran Using Salmonella typhimurium TA1535/pSK1002 umu Test
• PB113 SYNTHESIS AND BIOLOGICAL ACTIVITY OF β-D-GLUCOPYRANOSIDE OF THE MONOTERPENE ALCOHOLS
• Characterization of Volatile Aroma Compounds from Red and Black Rice Bran
• Species Differences in the Metabolism of (+)-and (-)-Limonenes and their Metabolites, Carveols and Carvones, by Cytochrome P450 Enzymes in Liver Microsomes of Mice, Rats, Guinea Pigs, Rabbits, Dogs, Monkeys, and Humans
• Volatile Flavor Components of ASTRAGALI RADIX (Astragalus membranaceus Bunge)(Organic Chemistry)
• Suppression of SOS-Inducing Activity of Chemical Mutagens by Yakuchinone A from Alpinia oxyphylla in the Salmonella typhimurium TA1535/pSK1002 umu Test
• The Novel Assay Method for Nicotine Metabolism to Cotinine Using High Performance Liquid Chromatography
• Inhibitory Compounds of α-Glucosidase Activity from Arctium lappa L.
• Identification of the Key Aroma Compounds in Dried Roots of Rubia cordifolia
• Biotransformation of Geraniol by the Larvae of Common Cutworm (Spodoptera litura)
• Use of Solid Phase Microextraction (SPME) for Profiling the Volatile Metabolites Produced by Glomerella cingulata
• Microbial Transformation of (-)-Isolongifolol by Plant Pathogenic Fungus Glomerella cingulata
• Influence of Root Exudates from Cucumis sativus and Daucus carota on Arbuscular Mycorrhizal Colonization
• Contractile and Extensile Effects of Red and White Wine on Rat and Mongolian gerbil Gastrointestinal Smooth Muscle
• Identification of the main aroma compounds in dried seeds of Brassica hirta
• A Useful Synthesis of Alkylidenesuccinic Acids by Wittig Reaction in Aqueous Media
• High-performance liquid chromatographic method for the determination of (-)-verbenone 10-hydroxylation catalyzed by rat liver microsomes
• Biotransformation of (-)-Verbenone by Human Liver Microsomes(Food & Nutrition Science)
• Synthesis and Characterization of New Ascorbic Derivative: Sodium Isostearyl 2-O-L-Ascorbyl Phosphate
• Chemical Composition of Volatile Oil from the Roots of Periploca sepium
• Biotransformation of (-)-Camphor by Salmonella typhimurium OY1002/2A6 Expressing Human CYP2A6 and NADPH-P450 Reductase
• Volatile Flavor Components of SINOMENI CAULIS ET RHIZOMA (Sinomenium acutum Rehder et Wilson)(Organic Chemistry)
• Volatile Flavor Components of PUERARIAE RADIX (Pueraria lobata Ohwi)(Organic Chemistry)
• Volatile Flavor Components of CORNI FRUCTUS (Cornus officinalis Sieb. et Zucc.)(Organic Chemistry)
• Preoperative 18[F]-fluorodeoxyglucose positron emission tomography/computed tomography predicts early recurrence after pancreatic cancer resection
• Biotransformation of Isoflavones by the Larvae of the Common Cutworm (Spodoptera litura)
• Components of the Essential Oil from Matteuccia struthiopteris
• In Vitro Metabolism of (-)-Camphor Using Human Liver Microsomes and CYP2A6(Biochemistry)
• Reduction of Oxazepam to Desmethyldiazepam by Human Intestinal Bacteria
• Components of Essential Oil from Rootstock of Coptis chinensis
• Components of Essential Oil from Rootstock of Coptis chinensis
• Microbial Transformation of Isopimpinellin by Glomerella cingulata
• Metabolism of (+)-Fenchone by CYP2A6 and CYP2B6 in Human Liver Microsomes(Biochemistry)
• Biotransformation of (-)-Dihydromyrcenol and (-)-Dihydromyrcenyl Acetate by the Larvae of Common Cutworm (Spodoptera litura)
• Metabolism of (+)-Terpinen-4-ol by Cytochrome P450 Enzymes in Human Liver Microsomes
• Quantitative Evaluation of (+)-.DELTA.3-Carene Metabolites from Living Larvae of Spodoptera litura by Headspace Solid-phase Microextraction
• Regeneration of extrahepatic bile ducts by tissue engineering with a bioabsorbable polymer
• Analysis of Volatile Organic Compounds Emitted by Plant Growth-Promoting Fungus Phoma sp. GS8-3 for Growth Promotion Effects on Tobacco

もっと見る 閉じる

スポンサーリンク