Identification of 1,3,6-Trinitropyrene as a Major Mutagen in Organic Extracts of Surface Soil from Nagoya City, Japan
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概要
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An organic extract from surface soil collected at a park in Nagoya, Aichi prefecture, which showed extremely high mutagenicity in Salmonella typhimurium TA98 in the absence of a mammalian metabolic system (S9 mix), was investigated to identify the major mutagens. A potent bacterial mutagen was isolated from the organic extract (1.03g) of the soil sample (2.1kg) by column chromatography. On the basis of mass spectra, the mutagen, which accounted for 8.8% of the mutagenicity of the soil extract, was thought to be a trinitrated polycyclic aromatic hydrocarbon with a molecular weight of m/z 337. The mutagen was synthesized from pyrene by nitration with nitric acid and was identified as 1,3,6-trinitropyrene (1,3,6-TNP) based on its ^1H NMR spectrum. The mutagenicity of 1,3,6-TNP in an Ames assay using S. typhimurium was extremely high, in that it induced 65,500 revertants/nmol in TA98, without S9 mix. This level of activity was slightly lower than that of 1,6-dinitropyrene (DNP), 129,800 revertants/nmol, or 1,8-DNP, 217,000 revertants/nmol, but was greater than that of 1,3-DNP, 4,260 revertants/nmol. These results indicate that 1,3,6-TNP was one of the major mutagens in surface soil collected at a park in Nagoya city, and this is the first report on the detection of 1,3,6-TNP in surface soil.
- 日本環境変異原学会の論文
- 2006-11-30
著者
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Watanabe Tetsushi
Department of Public Health, Kyoto Pharmaceutical University
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Hirayama Teruhisa
Department of Public Health, Kyoto Pharmaceutical University
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Mori Haruyuki
Department Of Drug Metabolism And Disposition Graduate School Of Pharmaceutical Sciences Nagoya City
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Takahashi Kazuhiko
Department of Drug Metabolism and Disposition, Graduate School of Pharmaceutical Sciences, Nagoya Ci
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Asanoma Masaharu
Nagoya City Public Health Research Institute
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Yoshida Satoshi
Department of Drug Metabolism and Disposition, Graduate School of Pharmaceutical Sciences, Nagoya Ci
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Gao Ning
Department of Drug Metabolism and Disposition, Graduate School of Pharmaceutical Sciences, Nagoya Ci
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Horibe Tomomi
Department of Drug Metabolism and Disposition, Graduate School of Pharmaceutical Sciences, Nagoya Ci
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Nukaya Haruo
School of Pharmaceutical Sciences, University of Shizuoka
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Mizutani Takaharu
Department of Drug Metabolism and Disposition, Graduate School of Pharmaceutical Sciences, Nagoya Ci
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Gao Ning
Department Of Drug Metabolism And Disposition Graduate School Of Pharmaceutical Sciences Nagoya City
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HIRAYAMA TERUHISA
Kyoto Pharmaceutical University
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Nukaya Haruo
School Of Pharmaceutical Science University Of Shizuoka
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Horibe Tomomi
Department Of Drug Metabolism And Disposition Graduate School Of Pharmaceutical Sciences Nagoya City
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Mizutani Takaharu
Department Of Drug Metabolism And Disposition Faculty Of Pharmaceutical Sciences Nagoya City Univers
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Watanabe Tetsushi
Department Of Correlative Study In Physics And Chemisty Graduate School Of Integrated Basic Sciences
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Hirayama Teruhisa
Department Of Orthopaedic Surgery Asahikawa Medical College
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Mizutani Takaharu
Department Of Drug Metabolism And Disposition Graduate School Of Pharmaceutical Sciences Nagoya City
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Takahashi Kazuhiko
Department Of Cardiovascular Diseases Miyagi Prefectural Semine Hospital
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Yoshida Satoshi
Department Of Drug Metabolism And Disposition Graduate School Of Pharmaceutical Sciences Nagoya City
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Yoshida Satoshi
Department Of Bimolecular Science Faculty Of Engineering Gifu University
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Hirayama Teruhisa
Kyoto College Of Pharmacy
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Takahashi Kazuhiko
Department Of Applied Chemistry Tokyo Institute Of Technology
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Takahashi Kazuhiko
Department Of Drug Metabolism And Disposition Graduate School Of Pharmaceutical Sciences Nagoya City
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