Determination of the Mutagen 3-Nitrobenzanthrone in Rainwater Collected in Kyoto, Japan

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概要

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• The mutagen 3-nitrobenzanthrone was determined in rainwater. Rainwater was collected at a residential area in Kyoto, Japan. Organic matter containing 3-nitrobenzanthrone in rainwater was extracted using a 3M (St. Paul, MN, U. S. A.) Empore Extraction Disk C_<18>, and the extract was purified on a pyrenylethyl silylated silica gel column. The purified sample was then reduced using a platinum black column heated at 80℃. The reduced product of 3-nitrobenzanthrone, i. e. 3-aminobenzanthrone, was analyzed by normal-phase HPLC with fluorescence detection. 3-Nitrobenzanthrone in the rainwater was determined in the range of 0.07-2.6ng 1^<-1>. The concentration was higher from May to July and lower from October to December. 1-Nitropyrene was also determined by reversed-phase HPLC with fluorescence detection after platinum black column reduction. 1-Nitropyrene was detected in the range of 0.0056-0.19 ng 1^<-1>, and this concentration range was lower than that of 3-nitrobenzanthrone.

• 2003-10-01

著者

• Watanabe Tetsushi

  Kyoto Pharmaceutical University

• HIRAYAMA TERUHISA

  Kyoto Pharmaceutical University

• MURAHASHI Tsuyoshi

  Kyoto Pharmaceutical University

• Iwanaga Erina

  Kyoto Pharmaceutical University

• Murahashi Tsuyoshi

  Kyoto Pharmaceutical University:(present Office)nihon Pharmaceutical University

• Hirayama Teruhisa

  Kyoto College Of Pharmacy

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