Mammalian Selenocysteine tRNA, Its Enzymes and Selenophosphate
スポンサーリンク
概要
- 論文の詳細を見る
Selenocysteine (Sec) is the 21st amino acid, because Sec has a specific tRNA and codon UGA, and shares a major stop codon UGA. The present article surveys the structure of mammalian tRNA^<Sec>, the identity elements, the enzymes to syntheize Sec, and selenophosphate as a Se donor. Sec-tRNA is converted from Ser-tRNA by Sec synthase. tRNA^<Sec> has a long 9bp AA-stem, 6bp D-stem and 4bp T-stem, differing from Ser tRNAs. The 9bp AA-stem and 6bp D-stem were identity elements necessary for selenylation, however, the 4bp T-stem is not essential. This finding was supported by the active mutants derived from major tRNA^<Ser> by changing the AA-stem to 9bp and the D-stem to 6bp. There are many enzymes and factors that produce and bring Sec-tRNA to ribosomes. The reaction proceeds as follows. The first step is Ser-tRNA synthetase which recognizes the discriminaition base G73 and the long extra arm. Bovine Sec synthase, composed of two protein species, recognizes Ser-tRNA^<Sec> among three Ser-tRNAs and changes Ser-tRNA^<Sec> to Sec-tRNA^<Sec>, by the addition of Se from selenophosphate (SeP). The stability of SeP and some properties of the synthetase are discussed. Sec-tRNA is recognized by an elongation factor specific to Sec-tRNA, and is brought to Se-protein mRNA. The discrimination mechanism of the Sec UGA codon from a major stop codon UGA remains to be resolved.
- 公益社団法人日本薬学会の論文
- 2000-12-01
著者
-
Mizutani Takaharu
Department Of Drug Metabolism And Disposition Faculty Of Pharmaceutical Sciences Nagoya City Univers
-
TOTSUKA Tsuyoshi
Department of Physiology, Institute for Developmental Research, Aichi Prefectural Colony
-
Goto Chiharu
Department of Drug Metabolism and Disposition, Faculty of Pharmaceutical Sciences, Nagoya City Unive
-
Goto Chiharu
Department Of Drug Metabolism And Disposition Faculty Of Pharmaceutical Sciences Nagoya City Univers
-
Totsuka Tsuyoshi
Department Of Physiology Aichi Prefectural Institute For Developmental Research
-
Totsuka Tsuyoshi
Department Of Embryology And Department Of Physiology Institute For Developmental Research Aichi Pre
関連論文
- Identification of 1,3,6-Trinitropyrene as a Major Mutagen in Organic Extracts of Surface Soil from Nagoya City, Japan
- The Influence of Quinolines on Coumarin 7- Hydroxylation in Bovine Liver Microsomes and Human CYP2A6
- EFFECTS OF REPETITIVE RECYCLING OF IMPULSE TRANSMISSION IN THE GASTROCNEMIUS OF NEOSTIGMINE TREATED RATS
- Different time courses of reduction in muscular potentials to moderate frequency stimulation in dystrophic and normal mice
- Mammalian Selenocysteine tRNA, Its Enzymes and Selenophosphate
- Inhibition Mechanism of UDP-Glucuronosyltransferase 1A6 by Xanthene Food Dyes
- In Vitro Activation of Valproate Glucuronidation by Carbapenem Antibiotics
- Bundles of Actin-Like Microfilaments Induced by Vinblastine in the Paraventricular Cells of the Rat Fetal Telencephalic Wall
- Substrate Specificity of Opioid Compounds to UDP-Glucuronosyltransferase (UGT), hUGT2B7 and Bovine Microsomal UGT
- Centronucleated Myofibers Having Also Peripheral Nuclei in Rectus Femoris Muscles of Muscular Dystrophic Mice
- Normal diameter distribution of tongue muscle fibers in muscular dystrophic mice. Consistent with the bone-muscle imbalance hypothesis for the pathogenesis.:Consistent with the Bone-Muscle Imbalance Hypothesis for the Pathogenesis
- Masking of a dystrophic symptom in genotypically dystrophic-dwarf mice.