Apoptosis of Human Melanoma Cells by a Combination of Lonidamine and Radiation
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概要
- 論文の詳細を見る
Human melanoma is the most aggressive form of human skin cancer, and is notoriously resistant to any current modalities of cancer therapy. Here we show that lonidamine (LND), a mitochondria-targeting nonconventional chemotherapeutic agent, markedly induced apoptosis in radioresistant human malignant melanoma C32TG cells. Either LND of up to 250 μM or X-ray irradiation of up to 15 Gy alone induced only a few percent of the apoptosis when administrated separately. When the two agents were combined, the apoptosis prominently increased to 29.3%. The apoptotic cells thus induced by the combination treatment showed chromatin condensation, a depletion in ΔΨm, and an activation of caspase-3. A pan-caspase inhibitor Z-Asp-CH_2DCB completely suppressed the apoptosis. The combination treatment also decreased Bcl-2 and Bad-phosphorylation. These results indicate that the mitochondria pathway of apoptosis would devise a new radiotherapy strategy for treating malignant melanoma.
- 日本放射線影響学会の論文
- 2004-06-15
著者
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ANDO Koichi
NIRS
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ANDO Koichi
Particle Therapy Radiation Biology Group, National Institute of Radiation Sciences
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ANDO Koichi
Laboratory of Heavy-ion Radiobiology for Therapy, National Institute of Radiological Science
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Miyato Yasuyuki
Heavy-ion Radiobiology Research Group National Institute Of Radiological Sciences
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Miyato Yasuyuki
Heavy-ion Radiobiology Research Group National Institute Of Radiological Sciences:graduate School Of
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Ando Koichi
Laboratory Of Heavy-ion Radiobiology For Therapy National Institute Of Radiological Science
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Ando Koichi
Heavy-ion Radiobiology Research Group National Institute Of Radiological Sciences:graduate School Of
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Ando Koichi
Heavy-ion Radio. Res. Nirs
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Ando Koichi
Clinical Radiation Biology And International Space Radiation Laboratory National Institute Of Radiol
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Ando Koichi
Dept. Of Radiology Kyoto Univ. : National Institute Of Radiological Sciences.
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