Cell Cycle Checkpoint and Apoptosis Induction in Glioblastoma Cells and Fibroblasts Irradiated with Carbon Beam
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概要
- 論文の詳細を見る
This study was conducted in order to evaluate the cytotoxicity of high linear-energy-transfer (LET) ionizing radiation (IR) on glioblastoma cells and fibroblasts using different modes of cell inactivation assays. Two human glioblastoma cell lines with or without p53-mutation, and fibroblasts were used as materials. Gamma rays and 290 MeV/u carbon beams with LET values of 20, 40, 80 keV/μm were used. To evaluate cell inactivation, we used colony formation assay, morphological detection of apoptosis, and flow-cytometry. Serial expressions of p53 and p21 were analyzed by immunoblotting. High-LET IR reduced the reproductive potency of these cells to identical levels in spite of differences in gammasensitivity, and yield of cell death correlated to LET values. A p53-wild- type glioblastoma cell line demonstrated a higher yield of apoptosis than other cell lines, whereas fibroblasts hardly displayed any cell death indicating senescence-like growth arrest even after high LET IR. A p53-mutant tumor cell line demonstrated very low yield of cell death with prominent G2/M arrest. Results of radiosensitivity differ according to what mode of cell inactivation is selected. While fibroblasts depend on G1 block after IR, G2/M blocks may play crucial roles in the radioresistance of p53-mutant glioblastoma cells.
- 日本放射線影響学会の論文
- 2007-07-15
著者
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TSUBOI Koji
Proton Medical Research Center, Graduate School of Comprehensive Human Sciences, University of Tsuku
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TOKUUYE Koichi
Proton Medical Research Center, University of Tsukuba
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ANDO Koichi
NIRS
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Tsuchida Yukihiro
Neurosurgery Faculty Of Medicine University Of Ryukyu
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Tsuboi Koji
Proton Medical Research Center Doctoral Program In Advanced Biomedical Applications Graduate School
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MORITAKE Takashi
Particle Therapy Radiation Biology Group, National Institute of Radiation Sciences
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MATSUMURA Akira
Neurosurgery, Doctoral Programs in Functional and Regulatory Medical Sciences, Graduate School of Co
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ANDO Koichi
Particle Therapy Radiation Biology Group, National Institute of Radiation Sciences
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Tokuuye Koichi
Proton Medical Research Center Doctoral Program In Advanced Biomedical Applications Graduate School
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Moritake Takashi
Particle Therapy Radiation Biology Group National Institute Of Radiation Sciences
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ANDO Koichi
Laboratory of Heavy-ion Radiobiology for Therapy, National Institute of Radiological Science
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Ando Koichi
Laboratory Of Heavy-ion Radiobiology For Therapy National Institute Of Radiological Science
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Ando Koichi
Particle Therapy Radiation Biology Group National Institute Of Radiation Sciences
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Ando Koichi
Clinical Radiation Biology And International Space Radiation Laboratory National Institute Of Radiol
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Tsuboi Koji
Proton Medical Research Center Doctoral Program In Advanced Biomedical Applications Graduate School
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Matsumura Akira
Neurosurgery Doctoral Programs In Functional And Regulatory Medical Sciences Graduate School Of Comp
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Ando Koichi
Dept. Of Radiology Kyoto Univ. : National Institute Of Radiological Sciences.
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