Cloning of a Complementary DNA for Auxin-Induced 1-Aminocyclopropane-1-carboxylate Synthase and Differential Expression of the Gene by Auxin and Wounding

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1-Aminocyclopropane-1-carboxylate (ACC) synthase induced by auxin was purified from slices of immature cucumber fruits, and partial amino acid sequences were determined. By using oligonucleotides synthesized based on the determined amino acid sequence, a complementary DNA for auxin-induced ACC synthase from winter squash (Cucurbita maxima Duch. cv Ebisu) was cloned and its sequence was determined. The sequence of the cDNA for the auxin-induced enzyme was markedly different from that for the wound-induced enzyme from the same plant [Nakajima et al. (1990) Plant Cell Physiol. 31: 1021], and the two cDNAs did not cross-hybridize. The results show that a gene for the auxin-induced enzyme (accA) is different from that for the wound-induced enzyme (accW). When hypocotyl sections of etiolated seedlings of winter squash were treated with IAA, the expression of accA was observed after 20 min and the level of mRNA increased maximally after 1 h, while only a small amount of the transcript of accW appeared. In wounded slices of mesocarp in which a large amount of mRNA for accW was present, the transcript of accA was not detected. We conclude that ACC synthase is encoded in two different genes which are expressed differentially by auxin and wounding. Possible biological significance of the presence of two genes for the enzyme is discussed.
日本植物生理学会の論文