Regulation of Auxin-induced Ethylene Biosynthesis : Repression of Inductive Formation of 1-Aminocyclopropane-1-carboxylate Synthase by Ethylene
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概要
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Changes in the 1-aminocyclopropane-1-carboxylate (ACC) synthase activity which regulates auxin-induced ethylene production were studied in etiolated mung bean hypocotyl segments. Increases both in ethylene production and ACC synthase activity in tissue treated with IAA and BA were severely inhibited by cycloheximide (CHI), 2-(4-methyl-2,6-dinitroanilino)-N-methylpropionamide, actinomycin D and α-amanitin. Aminoethoxyvinylglycine (AVG), a potent inhibitor of the ACC synthase reaction, increased the activity of the enzyme in the tissue 3- to 4-fold. This stimulation also was severely inhibited by the above inhibitors. Stimulation of the increase in the enzyme content by AVG was partially suppressed by an exogenous supply of ACC or ethylene. Suppression of the increase in the enzyme took place with 0.3 μl/liter ethylene, and inhibition was increased to 10μl/liter, which caused 65% suppression. Air-flow incubation of the AVG-treated tissue, which greatly decreased the ethylene concentration surrounding the tissue, further increased the amount of enzyme. Thus, one effect of AVG is to decrease the ethylene concentration inside the tissue. The apparent half life of ACC synthase activity, measured by the administration of CHI, was estimated as about 25 min. AVG lengthened the half life of the activity about 2-fold. Feedback repression by ethylene in the biosynthetic pathway of auxin-induced ethylene is discussed in relation to the effect of AVG.
- 日本植物生理学会の論文
著者
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Yoshii Hiroshi
Research Institute For Biochemical Regulation Faculty Of Agriculture Nagoya University
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Imaseki Hidemasa
Research Institute for Biochemical Regulation, Faculty of Agriculture, Nagoya University
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Imaseki Hidemasa
Research Institute For Biochemica Regulation Faculty Of Agriculture Nagoya University
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