Molecular Cloning and Sequence of a Complementary DNA Encoding 1-Aminocyclopropane-1-carboxylate Synthase Induced by Tissue Wounding
スポンサーリンク
概要
- 論文の詳細を見る
1-Aminocyclopropane-1-carboxylate (ACC) synthase [EC 4.4.1.14] is the key enzyme regulating ethylene biosynthesis in higher plants. A complementary DNA encoding wound-induced ACC synthase from mesocarp of winter squash (Cucurbita maxima Duch.) fruits was cloned, and its complete nucleotide sequence determined. The cloned cDNA contained an open reading frame of 1479 base pairs encoding a sequence of 493 amino acids. Identification of the cDNA was accomplished by expression of active enzyme in Escherichia coli harboring the cDNA and by the presence of a partial amino acid sequence identical to that found in the purified enzyme. A putative pyridoxal phosphate binding site of the enzyme is suggested. Northern blot analysis showed that the ACC synthase gene was activated by tissue wounding, and its expression was repressed by ethylene. Genomic Southern analysis indicates the presence of at least another sequence which weakly hybridizes with the cDNA.
- 日本植物生理学会の論文
著者
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Yamazaki Ken'ichi
Research Institute For Biochemical Regulation School Of Agricultural Sciences Nagoya University
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Mori Hitoshi
Agric. Sci. Nagoya Univ.
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Mori Hitoshi
Grad. Div. Biochem. Regulation Grad. Schl. Agricul. Sci. Nagoya Univ.
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Mori Hitoshi
Division Of Biological Regulation National Institute For Basic Biology
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Nakajima Nobuyoshi
Research Institute For Biochemical Regulation School Of Agriculture Nagoya University
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Mori H
National Inst. Basic Biology Okazaki Jpn
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Mori H
Nagoya Univ. Aichi Jpn
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Imaseki Hidemasa
Research Institute for Biochemical Regulation, Faculty of Agriculture, Nagoya University
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Mori Hitoshi
National Institute for Basic Biology
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Imaseki Hidemasa
Genetics Research Laboratory Japan Tobacco Inc.
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Imaseki Hidemasa
Grad. Div. Biochem. Regulation Grad. Schl. Agricul. Sci. Nagoya Univ.
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Imaseki Hidemasa
Research Institute For Biochemical Regulation. School Of Agricultural Sciences Nagoya University:div
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Imaseki Hidemasa
Research Institute For Biochemical Regulation School Of Agricultural Sciences Nagoya University:nati
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Imaseki Hidemasa
Division Of Biological Regulation National Institute For Basic Biology
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Imaseki Hidemasa
Nagoya University
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Imaseki Hidemasa
Research Institute For Biochemical Regulation School Of Agricultural Sciences Nagoya University:depa
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Yamazaki Ken-ichi
Department Of Microbial Genetics Research Institute For Microbial Disease Osaka University
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Imaseki H
Nagoya University
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Imaseki Hidemasa
Research Institute For Biochemica Regulation Faculty Of Agriculture Nagoya University
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