Molecular Size of Wound-Induced 1-Aminocyclopropane-1-carboxylate Synthase from Cucurbita maxima Duch. And Change of Translatable mRNA of the Enzyme after Wounding

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概要

• 論文の詳細を見る

• Wound-induced 1-aminocyclopropane-1-carboxylate (ACC) synthase was purified by an im-munoaffinity column from wounded mesocarp of winter squash (Cucurbita maxima Duch. cv. Ebisu) fruit, and a specific antibody was raised in rabbit. Translatable mRNA coding for ACC synthase was barely detectable in fresh tissue but clearly increased after wounding. The apparent molecular size of the purified enzyme as estimated by SDS-polyacrylamide gel electrophoresis (PAGE) was about 50 kDa. However, SDS-PAGE fluorograms of in vitro translation product of ACC synthase mRNA and the in vivo labeled enzyme as well as Western blot analysis showed that the subunit size of the enzyme was 58 kDa. The enzyme was partially degraded or processed to a 50 kDa peptide both in vivo and in vitro.

• 日本植物生理学会の論文

著者

• Nakagawa N

  Faculty Of Integrated Arts And Sciences Hiroshima University

• Nakagawa Naoki

  Osaka University

• Nakagawa Naoki

  Research Institute For Biochemical Regulation School Of Agriculture Nagoya University

• Nakajima N

  National Inst. Environmental Studies Tsukuba Jpn

• Nakajima Nobuyoshi

  Research Institute For Biochemical Regulation School Of Agriculture Nagoya University

• Imaseki Hidemasa

  Research Institute for Biochemical Regulation, Faculty of Agriculture, Nagoya University

• Imaseki Hidemasa

  Grad. Div. Biochem. Regulation Grad. Schl. Agricul. Sci. Nagoya Univ.

• Imaseki Hidemasa

  Research Institute For Biochemical Regulation. School Of Agricultural Sciences Nagoya University:div

• Imaseki Hidemasa

  Division Of Biological Regulation National Institute For Basic Biology

• Imaseki Hidemasa

  Nagoya University

• Imaseki Hidemasa

  Research Institute For Biochemical Regulation School Of Agricultural Sciences Nagoya University:depa

• Imaseki H

  Nagoya University

• Imaseki Hidemasa

  Research Institute For Biochemica Regulation Faculty Of Agriculture Nagoya University

• Nakagawa Naoki

  Research Institute For Biochemical Regulation School Of Agricultural Sciences Nagoya University

• Nakagawa N

  Hiroshima Univ. Higashi‐hiroshima Jpn

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