Enhancement of Reporter-Gene Expression by Insertions of Two Introns in Maize and Tobacco Protoplasts

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概要

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• The effects of two-intron insertions on reporter-gene expression were examined in transient assays in maize and tobacco. The first introns of a rice gene for phospholipase D (PLD), a maize gene for ubiquitin and a castor bean gene for catalase were tested with a gene for β-glucuronidase (GUS) or a gene for luciferase driven by a cauliflower mosaic virus 35S promoter (35S-GUS or 35S-Luc), the promoter of the maize ubiquitin (Ubi-GUS) or the promoter of a maize pyruvate, orthophosphate dikinase (PPDK-GUS). Although the enhancing effect varied, several intron combinations synergistically enhanced the reporters. When the first and second introns of the PLD gene were inserted in tandem, the reporter was synergistically enhanced. These results imply that expression enhancement by two introns may be observed in wide combinations of promoters, introns, and structural genes and that such enhancement plays certain roles in regulation of gene expression in higher plants.

• 日本植物細胞分子生物学会の論文
• 2004-03-01

著者

• KOMARI Toshihiko

  Japan Tobacco Inc.

• Komari Toshihiko

  Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc.

• Imaseki Hidemasa

  Genetics Research Laboratory Japan Tobacco Inc.

• Imaseki Hidemasa

  Grad. Div. Biochem. Regulation Grad. Schl. Agricul. Sci. Nagoya Univ.

• Imaseki Hidemasa

  Research Institute For Biochemical Regulation. School Of Agricultural Sciences Nagoya University:div

• Imaseki Hidemasa

  Research Institute For Biochemical Regulation School Of Agricultural Sciences Nagoya University:nati

• Imaseki Hidemasa

  Division Of Biological Regulation National Institute For Basic Biology

• Imaseki Hidemasa

  Nagoya University

• Imaseki Hidemasa

  Research Institute For Biochemical Regulation School Of Agricultural Sciences Nagoya University:depa

• Komari Toshihiko

  Plant Innovation Center Japan Tobacco Inc.

• UEKI Jun

  Plant Innovation Center, Japan Tobacco Inc.

• Imaseki H

  Nagoya University

• Ueki Jun

  Plant Innovation Center Japan Tobacco Inc.

• Komori Toshiyuki

  Genetics Research Laboratory Japan Tobacco Inc.

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