Improved Protocol for Transformation of Maize (Zea mays L.) Mediated by Agrobacterium tumefaciens
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概要
- 論文の詳細を見る
Maize inbred H99 and A188 generally respond well in tissue culture, but only A188 had been efficiently transformed by Agrobacterium tumefaciens by the method previously described. The problem in transformation of H99 appeared to be a negative impact of infection with A. tumefaciens. Various conditions and additives to the culture media to promote callus induction from infected immature embryos were screened. Two major modifications, 1) addition of silver nitrate to the media for selection of transformed cells and 2) use of carbenicillin in place of cefotaxime to eliminate bacteria after infection, turned out to be most effective. A large number of transgenic plants of H99 were produced by the improved protocol based on these modifications, whereas no transformants were created by the original protocol in these genotypes. Integration and inheritance of the transgenes were confirmed by Southern hybridization and genetic analysis. Even the frequency of transformation of A188 was further improved by the new protocol.
- 日本植物細胞分子生物学会の論文
- 2003-03-03
著者
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ISHIDA Yuji
Japan Tobacco Inc.
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KOMARI Toshihiko
Japan Tobacco Inc.
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Ishida Yuji
Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc.
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Komari Toshihiko
Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc.
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Ishida Y
Plant Innovation Center Japan Tobacco Inc.
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Komari Toshihiko
Plant Innovation Center Japan Tobacco Inc.
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Saito Hiroyoshi
Plant Breeding And Genetics Research Laboratory Japan Tobacco Inc.
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HIEI Yukoh
Plant Innovation Center, Japan Tobacco Inc.
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SAITO Hideaki
Applied Plant Research Center, Japan Tobacco Inc.
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Hiei Yukoh
Plant Innovation Center Japan Tobacco Inc.
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Komori Toshiyuki
Genetics Research Laboratory Japan Tobacco Inc.
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