Synthesis of an S-(2-Aminoethyl)-L-Cysteine Conjugate in S-(2-Aminoethyl)-L-Cysteine-Resistant Adenine-Auxotrophic Cells of Datura innoxia Mill

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A line of S-(2-aminoethyl)-L-cysteine-resistant adenine-auxotrophic cells (Ad^-AEC^r strain) was isolated from adenine-auxotrophic cells (Ad^- strain) of Datura innoxia Mill by a stepwise selection method. Ad^-AEC^r and B1 cells, which were cloned from the original Ad^-AEC^r cells, were able to grow actively on medium that contained 10 mM S-(2-aminoethyl)-L-cysteine (AEC), whereas the growth of Ad^- cells ceased completely in the presence of 0.5 mM AEC. The resistant phenotype has been maintained for at least 10 months in culture on medium without AEC. Levels of free lysine in Ad^-AEC^r and B1 cells were similar to that in Ad^- cells. By contrast, the level of free AEC in Ad^-AEC' cells was 10-fold lower than in Ad- cells and no free AEC was detectable in B1 cells. However, acid hydrolysis of extracts from Ad^-AEC^r and B1 cells resulted in a remarkable increase in levels of detectable AEC. This result indicates that conjugated AEC is synthesized and accumulated in the AEC-resistant cells. The level of the AEC conjugate in B1 cells increased with increases in the concentration of AEC in the culture medium, while intracellular levels of AEC were so low as not to be detectable in the case of cells grown on medium supplemented with AEC at less than I mM. The AEC conjugate was also detected in Ad^- cells, but at lower levels than in the AEC-resistant cells. In addition, AEC was found to be incorporated into soluble proteins in Ad^- cells. These results suggest that the resistance of AEC-resistant cells of Datura innoxia is accomplished via acceleration of the synthesis of the AEC conjugate which prevents any increase in intracellular levels of free AEC.
日本植物生理学会の論文

Synthesis of an S-(2-Aminoethyl)-L-Cysteine Conjugate in S-(2-Aminoethyl)-L-Cysteine-Resistant Adenine-Auxotrophic Cells of Datura innoxia Mill

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