Improved Co-transformation of Maize with Vectors Carrying Two Separate T-DNAs Mediated by Agrobacterium tumefaciens
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概要
- 論文の詳細を見る
Vectors with two separate T-DNAs for co-transformation mediated by Agrobacterium tumefaciens were tested in maize inbred A188. Although the frequency of transformation during the initial trials was very low, it was remarkably improved by the vector with a phosphinothricin resistance gene (bar) using a modified transformation protocol, which is characterized by the presence of silver nitrate and carbenicillin in the selection medium. The vector with hygromycin resistance gene (hpt) showed low frequency even with the improved protocol, but the use of maize ubiquitin gene promoter elevated the frequency. Both co-transformation and segregation of the progeny free from the selection markers frequencies were reasonably high and similar to those previously observed in rice and tobacco. The two-T-DNA vectors will be a useful tool in molecular biology and biotechnology studies of maize.
- 日本植物細胞分子生物学会の論文
- 2004-03-01
著者
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MURAI Nobuhiko
Plant Breed. Genet. Res. Lab., Japan Tobacco Inc.
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ISHIDA Yuji
Japan Tobacco Inc.
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OHTA Shozo
Japan Tobacco Inc.
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KOMARI Toshihiko
Japan Tobacco Inc.
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Saito Hideaki
Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc.
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Ishida Yuji
Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc.
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Ohta Shozo
Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc.
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Komari Toshihiko
Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc.
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Ohta S
Plant Breeding And Genetics Research Laboratory Japan Tobacco Inc.
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Ohta Shozo
Plant Breeding And Genetics Research Laboratory Japan Tobacco Inc.
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Ishida Y
Plant Innovation Center Japan Tobacco Inc.
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Murai Nobuhiko
Plant Breeding And Genetics Research Laboratory Japan Tobacco Inc.
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Komari Toshihiko
Plant Innovation Center Japan Tobacco Inc.
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Saito Hiroyoshi
Plant Breeding And Genetics Research Laboratory Japan Tobacco Inc.
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KURAYA Yoshiki
Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc.
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HIEI Yukoh
Plant Innovation Center, Japan Tobacco Inc.
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Hiei Yukoh
Plant Innovation Center Japan Tobacco Inc.
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Komori Toshiyuki
Genetics Research Laboratory Japan Tobacco Inc.
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Kuraya Yoshiki
Plant Breeding And Genetics Research Laboratory Japan Tobacco Inc.
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