Characterization of an Auxin-Inducible 1-Aminocyclopropane-1-Carboxylate Synthase Gene, VR-ACS6, of Mungbean (Vigna radiata(L.)Wilczek) and Hormonal Interactions on the Promoter Activity in Transgenic Tobacco
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概要
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A genomic clone for VR-ACS6, an isozyme of auxininducible ACC synthase of mungbean, was isolated, and its promoter activity was examined in transgenic tobacco. The clone contained 1, 612 bp long 5' untranscribed region and its coding sequence consisted of three exons and two introns. Genomic Southern hybridization indicated that VR-ACS6 is a single copy gene. The transcription initiation site was a cytosine present at 231-base upstream the translation start codon. The VR-ACS6 promoter contained DNA sequences homologous to various functionally identified auxin-responsive elements. To demonstrate hormonal response of the promoter region, transgenic tobacco plants carrying the 1, 719 bp VR-ACS6 promoter/-glucuronidase (GUS) fusion gene were generated. Strong GUS expression occurred by auxin treatment of leaves of T_0 transformants and hypocotyls of T_1 etiolated seedlings. Magnitude of the response to auxin was dose-dependent, and the increased GUS activity was detected at 0.1 μM and higher concentrations of IAA. Other plant hormones did not induce GUS activity, but greatly modified the response to auxin. Cytokinin enhanced the IAA-induced expression of GUS reporter gene, whereas ABA and ethylene suppressed the expression. These characteristics of VR-ACS6 promoter activity in transgenic tobacco are in good accordance with the expression patterns of the gene in mungbean hypocotyls. Histochemical staining showed that GUS activity was evident in both etiolated and light grown seedlings treated with IAA. Cytokinin enhanced the intensity of auxin-induced GUS stain and also expanded the stained area, whereas ABA and ethylene reduced both intensity and area of the stain.
- 日本植物生理学会の論文
著者
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Mori Hitoshi
Graduate School of Bioagricultural Sciences, Nagoya University
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Yoon In
Grad. Div. Biochem. Regulation Grad. Schl. Agricul. Sci. Nagoya Univ.
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Mori Hitoshi
Agric. Sci. Nagoya Univ.
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Mori Hitoshi
Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Mori Hitoshi
Grad. Div. Biochem. Regulation Grad. Schl. Agricul. Sci. Nagoya Univ.
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Mori Hitoshi
Division Of Biological Regulation National Institute For Basic Biology
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Mori H
National Inst. Basic Biology Okazaki Jpn
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Mori H
Nagoya Univ. Aichi Jpn
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Imaseki Hidemasa
Genetics Research Laboratory Japan Tobacco Inc.
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Imaseki Hidemasa
Grad. Div. Biochem. Regulation Grad. Schl. Agricul. Sci. Nagoya Univ.
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Imaseki Hidemasa
Research Institute For Biochemical Regulation. School Of Agricultural Sciences Nagoya University:div
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Imaseki Hidemasa
Research Institute For Biochemical Regulation School Of Agricultural Sciences Nagoya University:nati
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Imaseki Hidemasa
Division Of Biological Regulation National Institute For Basic Biology
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Imaseki Hidemasa
Nagoya University
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Imaseki Hidemasa
Research Institute For Biochemical Regulation School Of Agricultural Sciences Nagoya University:depa
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Park Don
Dept. Biol. Yonsei Univ.
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Kang B
Yonsei Univ. Seoul Kor
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Yoon In
Department of Biology, Yonsei University
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Park Don
Department of Biology, Yonsei University
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Imaseki Hidemasa
Graduate Division of Biochemical Regulation, School of Agricultural Sciences, Nagoya University
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Kang Bin.G.
Department of Biology, Yonsei University
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Imaseki H
Nagoya University
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Mori Hitoshi
Graduate School Of Bio-agricultural Sciences Nagoya University
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