Two Novel Transcripts Expressed in Pea Dormant Axillary Buds
スポンサーリンク
概要
- 論文の詳細を見る
To elucidate the molecular mechanism of apical dominance, the expression patterns of genes that are preferentially expressed in dormant axillary buds of pea(Pisum sativum L.cv.Alaska)seedlings were investigated. We isolated two cDNA clones, cPsAD1 and cPsAD2 whose corresponding genes were named PsAD1 and PsAD2, from a cDNA library of dormant axillary buds using the differential display method. The deduced amino acid sequence of PsAD1 contains 87 residues and is rich in glycine residues in the amino terminal region. A search of the protein databases failed to find any sequences similar to PsAD1 protein except for the glycine-rich region. Northern blot analyses showed that PsAD1 mRNA mainly accumulated in dormant axillary buds and that its amount rapidly decreased after decapitation of the terminal bud. In situ hybridization analyses indicated that PsAD1 mRNA was localized in the apical meristem, procambia, and leaf primordia in dormant axillary buds that were competent to grow out but whose growth was temporarily suspended. That is, the expression of the PsAD1 gene is closely associated with the dormancy of axillary buds. The deduced amino acid sequence of PsAD2 contains 98 amino acid residues and is not similar to those of previously characterized proteins. PsAD2 mRNA accumulated in dormant axillary buds, roots, mature leaflets and elongated stems, suggesting that PsAD2 is involved in not only the dormancy of axillary buds but also the non-growing state in various tissues.
- 日本植物生理学会の論文
著者
-
Mori Hitoshi
Graduate School Of Bioagricultural Sciences Nagoya Univ.
-
Madoka Y
Nagoya Univ. Nagoya Jpn
-
Madoka Yuka
Graduate School Of Bioagricultural Sciences Nagoya Univ.
-
Mori Hitoshi
Graduate School Of Bio-agricultural Sciences Nagoya University
関連論文
- Mapping of three QTLs that regulate internode elongation in deepwater rice
- A Major QTL Confers Rapid Internode Elongation in Response to Water Rise in Deepwater Rice
- Analysis of Cycles of Dormancy and Growth in Pea Axillary Buds Based on mRNA Accumulation Patterns of Cell Cycle-Related Genes
- EXPRESSION PATTERNS OF TUMOR SUPPRESSOR GENE(Rb) IN PEA AXILLARY BUDS
- Identification and cDNA Cloning of a Protein Abundantly Expressed during Apple Fruit Development
- Cloning of NAD-Dependent Sorbitol Dehydrogenase from Apple Fruit and Gene Expression
- An Isozyme of Betaine Aldehyde Dehydrogenase in Barley
- Expression of the Gene for NADP-Dependent Sorbitol-6-phosphate Dehydrogenase in Peach Leaves of Various Developmental Stages
- ACC SYNTHASE, LE-ACS2,IS ACTIVATED BY PHOSPHORYLATION OF A SERINE RESIDUE
- ANALYSIS OF TOUCH-INDUCIBLE ACC SYNTHASE ISOZYMES
- Rapid and Transient Expression of 1-Aminocyclopropane-1-Carboxylate Synthase Isogenes by Touch and Wound Stimuli in Tomato
- Characterization of an Auxin-Inducible 1-Aminocyclopropane-1-Carboxylate Synthase Gene, VR-ACS6, of Mungbean (Vigna radiata(L.)Wilczek) and Hormonal Interactions on the Promoter Activity in Transgenic Tobacco
- Identification and Characterization of a Gene Encoding Drought-Inducible Protein Localizing in the Bundle Sheath Cell of Sugarcane
- Ethylmethanesulfonate (EMS) mutagenesis of Solanum lycopersicum cv. Micro-Tom for large-scale mutant screens
- CLONING AND CHARACTERIZATION OF A cDNA ENCODING A DUNALIELLA TERTIOLECTA Ca^-DEPENDENT PROTEIN KINASE (CDPK)
- Molecular Identity of Uncoupling Proteins in Thermogenic Skunk Cabbage
- Male Flower-Specific Expression of Genes for Polygalacturonase, Pectin Methylesterase and β-1, 3-Glucanase in a Dioecious Willow(Salix gilgiana Seemen)
- A New Type of Endo-Xyloglucan Transferase Devoted to Xyloglucan Hydrolysis in the Cell Wall of Azuki Bean Epicotyls
- Acropetal Disappearance of PsAD1 Protein in Pea Axillary Buds after the Release of Apical Dominance
- LOCALIZATION OF AD1 WHICH SPECIFICALLY EXPRESSED IN DORMANT AXILLARY BUDS
- MANIPULATION OF AMOUNTS OF ACETYL-CoA CARBOXYLASE, THE KEY ENZYME IN FATTY ACID BIOSYNTHESIS
- Two Novel Transcripts Expressed in Pea Dormant Axillary Buds
- Changes in Protein Interactions of Cell Cycle-Related Genes during the Dormancy-to-Growth Transition in Pea Axillary Buds
- Abscisic acid-inducible 25 kDa xylem sap protein abundant in winter poplar