Rapid and Transient Expression of 1-Aminocyclopropane-1-Carboxylate Synthase Isogenes by Touch and Wound Stimuli in Tomato

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Touch stimuli induce ethylene production from 1-aminocyclopropane-1-carboxylic acid(ACC) in various plant species. We attempted to determine which ACC synthase(ACS) isogenes play a role in this phenomenon. We isolated cDNA clones(LE-ACS1A, 1B, 2, 3, 4 and 6) for ACC synthase isogenes from tomato, and their expressions were examined. Touch stimulation of tomato seedlings and fruits led to a large increase in the levels of LE-ACS6 and LE-ACS1A mRNA transcripts. The expressions of LE-ACS6 and LE-ACS1A isogenes increased within 10 min after touch, and mRNAs for both genes disappeared by 2h. Thus, their expressions are transient. Wounding of tomato leaves and fruits also gave rise to transient expressions of LE-ACS6 and LE-ACS1A. The mRNA of the LE-ACS2 gene, a wound-inducible gene, was detected after 2 h in wounded tissues, but not in touched tissues. The results clearly indicate that the two isogenes, LE-ACS6 and LE-ACS1A, sense only touch, contact or pressure, and that another isogene, LE-ACS2, requires severe cell damage for its expression.
日本植物生理学会の論文