The synthesis and properties of polysomal RNA in potato tuber slices during the early stage of aging

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The synthesis, molecular size, and coding properties of polysome-associated polyadenylated RNA [poly(A) (+)RNA] and non-polyadenylated RNA [poly(A) (-)RNA] were investigated in potato tuber discs during the early stage of aging. Tissue discs were labeled for 6 hr with ^3H-uridine in the presence of 5-fluorouracil to suppress rRNA synthesis, and polysomal RNA was isolated from the discs. Poly(A) (+)RNA accounted for 70% of the radioactivity in polysomal RNA and had a molecular size ranging from 6S to 30S with a peak at about 15S, when measured by formamide-polyacrylamide gel electrophoresis. The rest of the radioactivity was in poly(A) (-)RNA which had nearly the same range in molecular size, but had no conspicuous peaks on the gel. The polysomal RNA could program the synthesis of a wide variety of polypeptides in a cell-free translation system of wheat germ. Seventy percent of the translational capacity of polysomal RNA was attributed to poly(A) (+)RNA. The electrophoretic behaviour of the majority of the products from poly(A) (+)RNA was similar to that of products from poly(A) (-)RNA, but the former could program the synthesis of five polypeptides in addition to those translated from the latter. There was a tendency for poly(A) (-) RNA to be a more efficient messenger for large polypeptides.
日本植物生理学会の論文