Localization and Properties of Transcripts of psbA and rbcL Genes in the Stroma of Spinach Chloroplast

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A previous report detailed evidence that mRNA for the 32-kDa protein of PS II and mRNA for the large subunit of ribulosebisphosphate carboxylase/oxygenase (RuBisCO) are present in both the stroma and thylakoid of spinach chloroplast, and that the former mRNA in the stroma and the latter mRNA in the thylakoid fraction are not translated in situ [Minami and Watanabe (1984) Arch. Biochem. Biophys. 235: 562-570.]. Properties of the mRNA in stroma has been ex-amined by hybridization with probes of the respective genes after fractionation of stromal constituents by sucrose density gradient centrifugation. The results indicate that the transcripts of the psbA gene (mRNA for the 32-kDa protein) are not associated with ribosomes, whereas most of transcripts of the rbcL gene (mRNA for the large subunit of RuBisCO) are associated with polysomes in stroma. The extent of accumulation of these transcripts in the non-functioning sub-chloroplast fractions was determined by quantitative hybridization and was found to be unexpectedly large; close to half of the total population of transcripts of psbA, and more than half of that of rbcL were detected in stromal RNA and thylakoid-associated RNA, respectively. These findings are discussed in relation to the translational regulation of gene expression in the chloroplast.
日本植物生理学会の論文