RNA synthesis in the early stage of aerobic incubation of potato tuber discs

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RNA synthesis of potato tuber discs during the early period of their aerobic incubation was investigated by feeding the discs with ^3H-uridine. The rate of total RNA synthesis increased in two steps during the incubation. The increase during the first 2 to 3 hr was small, but that after 3 hr was large. The labeled RNAs were separated into poly(A) containing RNA [poly(A) (+) RNA] and poly(A) lacking RNA [poly(A) ( -) RNA] by the use of a poly(U)-Sepharose column. Poly(A) (+) RNA was synthesized even in the freshly prepared discs which incorporated little ^<14>C-leucine into a protein fraction, and the synthetic rate of poly(A) (+) RNA increased by about 50% during the first 3 hr incubation periods then gradually decreased thereafter. Synthesis of poly(A) (-) RNA continued to increase up to 7 hr after slicing. When the discs were pulse labeled, the proportion of radioactivity in poly(A) (+) RNA to that in the total RNA was maintained at about 50% until about 3 hr after slicing, but it abruptly decreased between 3 and 5 hr to about 35% which was maintained up to 9 hr after slicing.
日本植物生理学会の論文