7-Ethenyloxycoumarin as a New Substrate for Fluorophotometric Assay of Hepatic Microsomal Epoxidizing Activities
スポンサーリンク
概要
- 論文の詳細を見る
7-Ethenyloxycoumarin (7-vinyloxycoumarin, VOC) was metabolized by rat liver microsomes in the presence of a reduced nicotinamide adenine dinucleotide phosphate-generating system to 7-hydroxycoumarin (HOC) and glycolaldehyde via the unstable epoxide, 7-(epoxyethoxy) coumarin, as an obligatory intermediate which had a half life of 5.4 min in 0.1 M phosphate buffer, pH 7.4,at 37℃. The epoxide of VOC accumulated in the microsomal incubation mixture in the presence of the epoxide hydrolase inhibitor, 3,3,3-trichloropropene 1,2-oxide, was isolated and identified. HOC and glycolaldehyde were auto-decomposition products of the putative highly unstable intermediate, 7-(1', 2'-dihydroxyethoxy) coumarin, mostly formed by microsomal epoxide hydrolase from the epoxide. Direct fluorophotometry of HOC made it possible to determine epoxidizing activities of very small quantities of the microsomes from untreated rat liver (≥5μg protein). VOC was epoxidizied by rat liver microsomal cytochrome P-450,inducible by 3-methylcholanthrene (3-MC) and phenobarbital (PB), and the microsomal epoxidation reactions were inhibited by IgG preparations raised against the major cytochrome P-450 components isolated from 3-MC and PB-pretreated rat liver microsomes. In the untreated, 3-MC- and PB-pretreated rat liver microsomes, at least two monooxygenase components with different affinity were strongly suggested by a kinetic study, carried out using the antibodies, to be involved in the epoxidation of VOC.
- 公益社団法人日本薬学会の論文
著者
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WATABE Tadashi
Department of Molecular Toxicology, Nihon Pharmaceutical University
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SONE Tomomichi
Department of Toxicology, Faculty of Pharmaceutical Sciences, Setsunan University
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ISOBE Masakazu
Department of Toxicology, Faculty of Pharmaceutical Sciences, Setsunan University
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TAKABATAKE Eigo
Department of Toxicology, Faculty of Pharmaceutical Sciences, Setsunan University
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Watabe Tadashi
Department Of Drug Metabolism And Molecular Toxicology Tokyo University Of Pharmacy And Life Science
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Watabe T
Dept. Drug Metabolism And Molecular Toxicology School Of Pharmacy Tokyo University Of Pharmacy And L
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Ozawa Naoki
Toxicology And Efficacy Research Tsukuba Research Laboratories Pharmacia And Upjohn
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Ozawa Naoki
Pharmacia Waseda University
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Ozawa Naoki
Department Of Toxicology Faculty Of Pharmaceutical Sciences Setsunan University:laboratory Of Drug M
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Ozawa Naoki
Department Of Hygienic Chemistry Tokyo College Of Pharmacy
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Ozawa Naoki
Drug Metabolism And Analytical Chemistry Research Upjohn Pharmaceuticals Limited Tsukuba Research La
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Sone T
Osaka Univ. Osaka Jpn
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Sone Tomomichi
Department Of Toxicology Faculty Of Pharmaceutical Sciences Setsunan University
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Sone Tomomichi
Laboratory Of Drug Metabolism And Toxicology Department Of Hygienic Chemistry Tokyo College Of Pharm
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Isobe M
Setsunan Univ. Osaka Jpn
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Isobe M
Department Of Toxicology Faculty Of Pharmaceutical Sciences Setsunan University
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Isobe Masakazu
Department Of Toxicology Faculty Of Pharmaceutical Sciences Setsunan University
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Isobe Masakazu
Faculty Of Pharmaceutical Sciences University Of Setsunan
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Takabatake E
Setsunan Univ. Osaka Jpn
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Sone Tomomichi
Faculty Of Pharmaceutical Sciences Setsunan University
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Takabatake Eigo
Department Of Pharmaceutical Sciences Institute Of Public Health
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Tanaka E
Univ. Tsukuba Ibaraki Jpn
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Sone T
Setsunan Univ. Osaka Jpn
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Watabe Tadashi
Department Of Toxicology Faculty Of Pharmaceutical Sciences Setsunan University:laboratory Of Drug M
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Isobe Masakazu
Laboratory Of Drug Metabolism Ε-toxicology Department Of Hygienic Chemistry Tokyo College Of Pharmac
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Takabatake E
Inst.of Commun.med
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Tanaka Einosuke
Inst.of Commun.med
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Watabe Tadashi
Laboratory Of Drug Metabolism And Toxicology Department Of Hygienic Chemistry Tokyo College Of Pharm
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Watabe Tadashi
Department Of Drug Metabolism And Molecular Toxicology School Of Pharmacy Tokyo University Of Pharma
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OZAWA Naoki
Department of Biochemical Pharmacology, Faculty of Pharmaceutical Sciences, Chiba University
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