p-NITROPHENYL VINYL ETHER, A NOVEL SUBSTRATE FOR THE ASSAY OF CYTOCHROME P-450 DEPENDENT OLEFINIC EPOXIDATION IN HEPATIC MICROSOMES
スポンサーリンク
概要
- 論文の詳細を見る
p-Nitrophenyl vinyl ether (NPVE) was metabolized to p-nitrophenol and glycolaldehyde via an epoxide by rat hepatic microsomes. The cofactor requirment and effects of monooxygenase inhibitors indicated that the oxidative metabolism of NPVE was mediated by microsomal cytochrome P-450. Epoxide hydrolase plays a minor role, because a strong epoxide hydrolase inhibitor, 3,3,3-trichloropropene oxide, showed a weak inhibitory effect on the p-nitrophenol formation. The epoxy intermediate is so labile that the hydrolysis of the epoxide proceeds mostly nonenzymically even at a neutral pH. Induction experiments suggested that NPVE was susceptible to a wide varieties of cytochrome P-450 species. Thus, a convenient and sensitive method for the assay of olefinic epoxidase activity in hepatic microsomes was developed with NPVE as a substrate.
- 公益社団法人日本薬学会の論文
著者
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SONE Tomomichi
Department of Toxicology, Faculty of Pharmaceutical Sciences, Setsunan University
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ISOBE Masakazu
Department of Toxicology, Faculty of Pharmaceutical Sciences, Setsunan University
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TAKABATAKE Eigo
Department of Toxicology, Faculty of Pharmaceutical Sciences, Setsunan University
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Sone T
Osaka Univ. Osaka Jpn
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Sone Tomomichi
Department Of Toxicology Faculty Of Pharmaceutical Sciences Setsunan University
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Sone Tomomichi
Laboratory Of Drug Metabolism And Toxicology Department Of Hygienic Chemistry Tokyo College Of Pharm
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Isobe M
Setsunan Univ. Osaka Jpn
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Isobe M
Department Of Toxicology Faculty Of Pharmaceutical Sciences Setsunan University
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Isobe Masakazu
Department Of Toxicology Faculty Of Pharmaceutical Sciences Setsunan University
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Isobe Masakazu
Faculty Of Pharmaceutical Sciences University Of Setsunan
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Takabatake E
Setsunan Univ. Osaka Jpn
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Sone Tomomichi
Faculty Of Pharmaceutical Sciences Setsunan University
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Takabatake Eigo
Department Of Pharmaceutical Sciences Institute Of Public Health
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Sone T
Setsunan Univ. Osaka Jpn
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Isobe Masakazu
Laboratory Of Drug Metabolism Ε-toxicology Department Of Hygienic Chemistry Tokyo College Of Pharmac
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Takabatake E
Inst.of Commun.med
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