マウスにおける薬物の脳室内注入法

概要

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A method has been described for studies of the central effects of drugs by intraventricular injection to the unrestrained conscious mouse. The site of the chronic cannulation was 3.5 mm caudal from the inferior cerebral vein and 1 mm lateral from the sagittal suture. A stainless steel cannula (0.8 mm diameter) held in the carrier of a stereotaxic instrument was inserted vertically into the brain at a depth of 3 mm, and 0.005 to 0.01 ml of drug solution was administered through a needle which had been inserted into the cannula and connected at the opposite end to a microinjector through a flexible plastic tube. Immediately after the intraventricular injection of methamphetamine (MA), methylphenidate (MP), and caffeine sodium benzoate (C.S.B.), an increased rotation ratio and a decreased center-passing ratio were obsereved as in the case of the subcutaneous injection of MA and MP. After subcutaneous administration of C.S.B., however, the center-passing ratio increased remarkably but the rotation ratio did not increase significantly.
公益社団法人日本薬学会の論文
1968-02-25