ヒト絨毛性ゴナドトロビンの免疫学的測定法の研究(第2報)抗体感作ラテックス凝集反応試薬の調製条件

概要

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Fundamental examinations were made on preparative conditions for the anti-human chorionic gonadotropin (HCG)-antibody-coated latex slide test for the immunological assay of HCG by using the rabbit antibody to immunologically homogeneous HCG. The relation between the antibody titer for HCG in the hemagglutination reaction, and the sensitivity and prozone phenomenon in the latex slide test indicated that the sensitivity and prozone became better as the antibody titer became higher. The relation between the diameter of latex particles and the sensitivity and prozone phenomenon indicated that the sensitivity becomes higher as the latex particle diameter becomes larger and, inversely, the prozone became lower with lowering of the sensitized amount of optimum anti-HCG-immunoglobulin G (IgG). However, amount of sensitized IgG per unit area of latex particles that showed optimum sensitivity and prozone gave a constant value (214-223 ng/cm^2) even though the particle diameter was different. In the agglutination reaction between HCG and human luteinizing hormone (HLH) and human thyroid-stimulating hormone (HTSH), a crossreaction was observed when the sensitivity of the latex slide test for HCG was 0.6-1.0 IU/ml, at 1.7-4.0 IU/ml in HLH, and at 10-19 mUSP units/ml in HTSH.
公益社団法人日本薬学会の論文
1978-03-25