ヒト絨毛性ゴナドトロビンの免疫学的測定法の研究(第1報)精製とその免疫学的および物理化学的性質

概要

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In order to obtain a large amount of immunologically homogeneous human chorionic gonadotropin (HCG), a method for its purification was devised, HCG of 5550 IU/mg (immunological activity) was obtained in 29% yield from a commercial crude HCG (immunological activity ; 1550 IU/mg) by using column chromatography with DEAE-Sephadex A-25,Con. A-Sepharose, and Sephadex G-100. The purified HCG appeared to be homogeneous by the Ouchterlony immunodiffusion, immunoelectrophoresis, and ultracentrifugation. The S_<20,W> value was found to be 3.07 S. The Stokes'radius of HCG was determined as 37Å by gel filtration on Sephadex G-150. The amino acid composition and the content of carbohydrate were approximately equal to the values of other purified samples, taking the biological activity as an index. However, when analysis by disc electrophoresis revealed two bands close to each other and, analysis by isoelectrofocusing showed peakes of high specific activity at pH 3.90 and 4.05.
公益社団法人日本薬学会の論文
1978-03-25