高速液体クロマトグラフィーによる化粧品中のグアイアズレンの定量法

概要

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A procedure is described for the determination of guaiazulene (GA) in cosmetics by high performance liquid chromatography (HPLC). The sample was mixed with Cellite, transfered to a chromatographic column, and eluted with acetonitrile. The acetonitrile was added to water, and liquid-liquid partition between hexane. The hexane was concentrated and then GA was determined by HPLC on octadecylsilated silica column using a mobile phase of methanol-water (9 : 1,v/v), UV detector (284 nm) and fluorescence spectrophotometric detector (FD) (Ex・λ : 285 nm, Em・λ : 390 nm) for detection. As a result, the calibration curve was linear in the range 1-10 μg/ml of GA on FD and UV detector. Recoveries of GA obtained from a variety of cosmetic samples spiked at 50 and 100 ppm levels were 93.8-97.2% and 94.8-97.0%, respectively. When this method was applied to the analysis of GA in commercial samples, GA could be determined without the influence of obstructive substances.
公益社団法人日本薬学会の論文
1984-06-30